Schwarz M A, Juliano R L
J Cell Physiol. 1985 Jul;124(1):113-9. doi: 10.1002/jcp.1041240118.
Fibronectin (Fn) was adsorbed onto neutral, sulfonated, imine-conjugated or gelatin coated polystyrene latex beads. In all cases, the Fn coated beads bound effectively to Chinese hamster ovary (CHO) cells in suspension. However, the binding of Fn coated neutral or positively charged imine conjugated bead was inhibited by low concentrations of heparin or heparan sulfate or by treatment of the cells with Flavobacterium heparanase. By contrast, binding of Fn coated sulfonated or gelatin beads was insensitive to inhibition by heparin and to heparanase treatment of cells. Adhesion of CHO cells to Fn coated tissue culture plastic was not sensitive to heparin, whereas adhesion of CHO cells to Fn-coated imine-conjugated plastic was sensitive to heparin. These observations imply that the functional status of Fn can be modulated by the nature of the surface to which the Fn is adsorbed. They further imply that, under some circumstances, the heparin/heparan sulfate binding domains of Fn can play a role in the attachment of Fn to the cell membrane via membrane proteoglycans. Under other circumstances, the interaction of Fn with the cell may primarily involve other receptors for Fn, presumably cell surface glycoproteins.
纤连蛋白（Fn）被吸附到中性、磺化、亚胺共轭或明胶包被的聚苯乙烯乳胶珠上。在所有情况下，包被有Fn的珠子都能有效地与悬浮状态的中国仓鼠卵巢（CHO）细胞结合。然而，包被有Fn的中性或带正电荷的亚胺共轭珠的结合会被低浓度的肝素或硫酸乙酰肝素抑制，或者通过用肝素黄杆菌酶处理细胞来抑制。相比之下，包被有Fn的磺化或明胶珠的结合对肝素抑制以及对细胞进行肝素酶处理不敏感。CHO细胞与包被有Fn的组织培养塑料的黏附对肝素不敏感，而CHO细胞与包被有Fn的亚胺共轭塑料的黏附对肝素敏感。这些观察结果表明，Fn的功能状态可被Fn所吸附的表面性质所调节。它们还进一步表明，在某些情况下，Fn的肝素/硫酸乙酰肝素结合结构域可通过膜蛋白聚糖在Fn与细胞膜的附着中发挥作用。在其他情况下，Fn与细胞的相互作用可能主要涉及Fn与其他受体的相互作用，推测是细胞表面糖蛋白。
