Li Chao, Wu Xiaoyuan, Wang Pengna, Wang Hongru, Wang Lidong, Sun Fang, Lu Cheng, Hao Huaiqing, Chu Chengcai, Jing Hai-Chun
Engineering Laboratory for Grass-based Livestock Husbandry, Institute of Botany, Chinese Academy of Sciences, Beijing, 100093, China.
University of Chinese Academy of Sciences, Beijing, 100049, China.
J Exp Bot. 2025 Jan 10;76(2):331-345. doi: 10.1093/jxb/erae391.
Darkness is often used as an effective measure to induce leaf senescence. Although many senescence-related genes in rice have been reported, the genome-wide genetic architecture underlying leaf senescence remains poorly understood. In our study, indica and japonica rice showed contrasting responses to dark-induced leaf senescence (DILS). Genome-wide association studies (GWAS) combined with transcriptomic analyses revealed 57, 97, and 48 loci involved in the regulation of the onset, progression, and ending of DILS, respectively. Haplotype analyses showed that the senescence-related loci differentially accumulated in indica and japonica accessions and functioned additively to regulate DILS. A total of 357 candidate genes were identified that are involved in various senescence-related processes such as lipid and amino acid catabolism, photosynthesis, response to reactive oxygen species, and regulation of defence response. In addition, functional analyses of candidate genes revealed that OsMYB21 positively regulates the onset of DILS, while OsSUB1B negatively regulates its progression. Thus, our results provide new insights into the genetic regulation of DILS in rice.
黑暗常被用作诱导叶片衰老的有效手段。尽管已经报道了水稻中许多与衰老相关的基因，但叶片衰老背后的全基因组遗传结构仍知之甚少。在我们的研究中，籼稻和粳稻对黑暗诱导的叶片衰老（DILS）表现出相反的反应。全基因组关联研究（GWAS）结合转录组分析分别揭示了57个、97个和48个参与调控DILS起始、进程和结束的位点。单倍型分析表明，衰老相关位点在籼稻和粳稻品种中差异积累，并以累加方式调控DILS。共鉴定出357个候选基因，它们参与各种衰老相关过程，如脂质和氨基酸分解代谢、光合作用、对活性氧的反应以及防御反应的调控。此外，对候选基因的功能分析表明，OsMYB21正向调控DILS的起始，而OsSUB1B负向调控其进程。因此，我们的结果为水稻中DILS的遗传调控提供了新的见解。
