Fichelson S, Heard J M, Levy J P
J Cell Physiol. 1985 Sep;124(3):487-92. doi: 10.1002/jcp.1041240319.
To investigate the mechanisms supporting the in vitro longterm growth of murine leukemic myeloblastic cells, factor-dependent and autonomous myeloblastic cells have been examined for their CSF responsiveness, CSF secretion, and autostimulation of growth. Purified CSF-1, GM-CSF, and IL-3 stimulated cloning and proliferation of both autonomous (ACL) and factor-dependent cell lines (FDCL), and were unable to induce differentiation of these cells. Sensitivities to CSFs were similar for ACL, FDCL and normal bone marrow cells. Most of the cell lines secreted CSFs, stimulating colony formation from normal bone marrow cells in bilayer agar cultures. The number of induced granulo-macrophagic colonies was similar in the presence of either ACL or FDCL. The ability to stimulate their own proliferation was similar for both ACL and FDCL in a longterm suspension culture assay as well as in a shortterm cloning assay. These results strongly suggest that the autocrine secretion of CSF-related activities are not sufficient to fully account for the in vitro longterm proliferation of virus-transformed myelomonocytic cells.
为了研究支持小鼠白血病髓母细胞体外长期生长的机制，对因子依赖性和自主性髓母细胞进行了集落刺激因子（CSF）反应性、CSF分泌及生长自刺激方面的检测。纯化的CSF-1、粒细胞-巨噬细胞集落刺激因子（GM-CSF）和白细胞介素-3（IL-3）刺激自主性（ACL）和因子依赖性细胞系（FDCL）的克隆和增殖，且不能诱导这些细胞分化。ACL、FDCL和正常骨髓细胞对CSF的敏感性相似。大多数细胞系分泌CSF，在双层琼脂培养中刺激正常骨髓细胞形成集落。在ACL或FDCL存在的情况下，诱导的粒-巨噬细胞集落数量相似。在长期悬浮培养试验以及短期克隆试验中，ACL和FDCL刺激自身增殖的能力相似。这些结果有力地表明，CSF相关活性的自分泌不足以完全解释病毒转化的髓单核细胞的体外长期增殖。
