Yamamoto K, Niwa A, Yasumura Y
J Cell Physiol. 1985 Oct;125(1):91-7. doi: 10.1002/jcp.1041250112.
A choline non-required cell line was established from a rat hepatoma cell line. The line designated R-Y121B X cho was able to grow in choline-deprived medium without serum and lipid. Choline is necessary for the biosynthesis of phosphatidylcholine, which is a main component of cell membranes. Phosphatidylcholine can be synthesized by the methylation of phosphatidylethanolamine in liver cells. Phospholipid composition and incorporation of radiolabeled serine into phosphatidylserine or phosphatidylethanolamine were quite similar in R-Y121B X cho and its parental cells. However R-Y121B X cho cells had higher phosphatidylcholine synthesis activity from radiolabeled methionine than parental cells. These results indicate that choline requirement of mammalian cells depends on the activity of phosphatidylethanolamine methyltransferase.
从大鼠肝癌细胞系中建立了一种无需胆碱的细胞系。命名为R-Y121B X cho的该细胞系能够在无血清和脂质的胆碱缺乏培养基中生长。胆碱是细胞膜的主要成分磷脂酰胆碱生物合成所必需的。磷脂酰胆碱可通过肝细胞中磷脂酰乙醇胺的甲基化合成。R-Y121B X cho及其亲本细胞的磷脂组成以及放射性标记的丝氨酸掺入磷脂酰丝氨酸或磷脂酰乙醇胺的情况非常相似。然而，R-Y121B X cho细胞从放射性标记的甲硫氨酸合成磷脂酰胆碱的活性比亲本细胞高。这些结果表明，哺乳动物细胞对胆碱的需求取决于磷脂酰乙醇胺甲基转移酶的活性。
