牛泰勒虫裂殖子生物合成标记单克隆抗体在腹水中的产生

Production in ascites fluid of biosynthetically labelled monoclonal antibody to Theileria parva sporozoites.

作者信息

Dobbelaere D A, Spooner P R

出版信息

J Immunol Methods. 1985 Oct 10;82(2):209-14. doi: 10.1016/0022-1759(85)90352-7.

Abstract

A hybridoma cell line has been previously produced which secretes monoclonal antibodies able to neutralize sporozoites of Theileria parva, the causative agent of East Coast fever of cattle. Cells from this line were injected intra-peritoneally into pristane-treated BALB/c mice. During the last 4 days of hybridoma cell growth, mice were given 4 daily intraperitoneal injections of a mixture of tritiated amino acids in order to biosynthetically radiolabel the monoclonal antibody being produced in ascites fluid. The specific activity of the antibody obtained was 100 mCi/mmol. The labelled antibody was used to detect, by autoradiography, a surface coat antigen of T. parva sporozoites in cryostat sections of Theileria-infected tick salivary glands. The method allows the preparation of large quantities of biosynthetically radiolabelled immunological probes for the detection of immunoreactive sites in biological specimens.

摘要

先前已制备出一种杂交瘤细胞系，该细胞系分泌的单克隆抗体能够中和牛东海岸热的病原体——小泰勒虫的子孢子。将该细胞系的细胞腹腔注射到经 pristane 处理的 BALB/c 小鼠体内。在杂交瘤细胞生长的最后 4 天，每天给小鼠腹腔注射 4 次氚标记氨基酸混合物，以便通过生物合成对腹水中产生的单克隆抗体进行放射性标记。所获得抗体的比活度为 100 mCi/mmol。通过放射自显影，用标记抗体在小泰勒虫感染的蜱唾液腺的低温切片中检测小泰勒虫子孢子的表面被膜抗原。该方法可制备大量用于检测生物标本中免疫反应位点的生物合成放射性标记免疫探针。