Department of Biomedical Engineering, Huazhong University of Science and Technology, Wuhan 430074, PR China.
Department of Botany and Microbiology, College of Science, Al-Azhar University, Assiut Branch, Assiut 71524, Egypt.
Int J Biol Macromol. 2024 Nov;280(Pt 2):135668. doi: 10.1016/j.ijbiomac.2024.135668. Epub 2024 Sep 19.
The demand for advanced biomaterials in medical treatments is rapidly expanding. To address this demand, a nanocomposite of cellulose nanofiber (CNF) with chitosan (Ch) and graphene oxide (GO) was developed for burn wound treatment. The CNF-Ch-GO nanocomposites were characterized and their biological properties were evaluated. Microscopic images showed a uniform distribution of CNF, Ch, and GO with a porous structure. ATR-FTIR and XRD analyses confirmed the chemical structures, while a thermogravimetric study confirmed the stability of CNF-Ch-GO nanocomposite under a N atmosphere. The synthesized CNF-Ch-GO nanocomposite exhibited rapid absorption, absorbing 1781.7 ± 53.7 % PBS in 2 min. It demonstrated a Young's modulus of 11.90 ± 0.06 MPa in a hydrated condition, indicating its mechanical stability in water. Furthermore, it displayed excellent biocompatibility and hemocompatibility with 96.23 ± 12.21 % cell viability and 0.21 ± 0.08 % of hemolysis. Additionally, the blood clotting index of CNF-Ch-GO was comparable to that of standard dressing gauze. To enhance antimicrobial efficacy, CNF-Ch-GO was conjugated with lysozyme. This biotic and abiotic conjugation resulted in 92.17 % ± 3.02 % and 94.99 ± 2.1 % eradication of Escherichia coli and Staphylococcus aureus, respectively. The enhanced antimicrobial properties, biocompatibility, and mechanical stability of the superabsorbent CNF-Ch-GO nanocomposite indicate its significant potential for advanced burn wound healing applications.
对高级生物材料在医疗中的应用的需求正在迅速扩大。为了满足这一需求，开发了一种纤维素纳米纤维（CNF）与壳聚糖（Ch）和氧化石墨烯（GO）的纳米复合材料，用于烧伤创面治疗。对 CNF-Ch-GO 纳米复合材料进行了表征，并评估了其生物学性能。显微镜图像显示 CNF、Ch 和 GO 具有均匀的分布和多孔结构。ATR-FTIR 和 XRD 分析证实了化学结构，而热重研究证实了 CNF-Ch-GO 纳米复合材料在 N 气氛下的稳定性。合成的 CNF-Ch-GO 纳米复合材料表现出快速吸收能力，在 2 分钟内吸收 1781.7 ± 53.7%的 PBS。在水合状态下，其杨氏模量为 11.90 ± 0.06 MPa，表明其在水中具有机械稳定性。此外，它表现出优异的生物相容性和血液相容性，细胞存活率为 96.23 ± 12.21%，溶血率为 0.21 ± 0.08%。此外，CNF-Ch-GO 的凝血指数与标准敷料纱布相当。为了增强抗菌效果，将 CNF-Ch-GO 与溶菌酶结合。这种生物和非生物的结合分别导致大肠杆菌和金黄色葡萄球菌的消除率达到 92.17%±3.02%和 94.99%±2.1%。高吸水性 CNF-Ch-GO 纳米复合材料具有增强的抗菌性能、生物相容性和机械稳定性，表明其在高级烧伤创面愈合应用中具有重要的应用潜力。
