Lutalo-Bosa A J, Adolphson J L, Albers J J
J Lipid Res. 1985 Aug;26(8):995-1001.
Radial immunodiffusion (RID) has been used for determination of low density lipoprotein (LDL) B protein in plasma. During measurement of B protein in plasma and the d less than and d greater than 1.019 g/ml plasma fractions by RID in 1.0%, 1.5%, 2.0%, and 2.5% agarose, the d less than 1.019 g/ml lipoproteins diffuse in the agarose and produce precipitin rings. Among normotriglyceridemic subjects, the B protein values in whole plasma obtained by RID using 1.5 to 2.5% agarose were only slightly higher than the values in the d greater than 1.019 g/ml fraction obtained by RID and closely approximated the values obtained in the d greater than 1.019 g/ml fraction by radioimmunoassay. However, among the hypertriglyceridemic subjects, the RID measurement of B protein in plasma using 1.0 to 2.5% agarose overestimated the LDL B protein levels in plasma. The RID procedure at agarose concentrations of 1.5% to 2.5% can be used to estimate plasma LDL B protein levels in normotriglyceridemic subjects. However, measurement of LDL B protein by RID in plasma of hypertriglyceridemic subjects must be interpreted with caution; the LDL B protein is overestimated by this procedure because of the contribution by the d less than 1.019 g/ml lipoproteins to the B protein value.
放射免疫扩散法（RID）已用于测定血浆中的低密度脂蛋白（LDL）B蛋白。在使用1.0%、1.5%、2.0%和2.5%琼脂糖通过RID测定血浆中B蛋白以及密度小于和大于1.019 g/ml的血浆组分时，密度小于1.019 g/ml的脂蛋白在琼脂糖中扩散并产生沉淀环。在正常甘油三酯血症受试者中，使用1.5%至2.5%琼脂糖通过RID获得的全血浆B蛋白值仅略高于通过RID在密度大于1.019 g/ml组分中获得的值，并且与通过放射免疫测定在密度大于1.019 g/ml组分中获得的值非常接近。然而，在高甘油三酯血症受试者中，使用1.0%至2.5%琼脂糖通过RID测定血浆中的B蛋白会高估血浆中的LDL B蛋白水平。琼脂糖浓度为1.5%至2.5%的RID程序可用于估计正常甘油三酯血症受试者的血浆LDL B蛋白水平。然而，在高甘油三酯血症受试者的血浆中通过RID测定LDL B蛋白时必须谨慎解释；由于密度小于1.019 g/ml的脂蛋白对B蛋白值的贡献，该程序会高估LDL B蛋白。
