Department of Veterinary Pharmacology and Toxicology, National Key Laboratory of Veterinary Public Health Security, Beijing Key Laboratory of Detection Technology for Animal-Derived Food Safety, Beijing Laboratory for Food Quality and Safety, College of Veterinary Medicine, China Agricultural University, Beijing, 100193, People's Republic of China.
Department of Chemistry, Waterloo Institute for Nanotechnology, University of Waterloo, Waterloo, Ontario, N2L 3G1, Canada.
Biosens Bioelectron. 2025 Jan 1;267:116797. doi: 10.1016/j.bios.2024.116797. Epub 2024 Sep 18.
The development of more sensitive, stable, and portable biosensors is crucial for meeting the growing demands of diverse and complex detection environments. MOF-based nanozymes have emerged as excellent optical reporters, making them ideal signal donors for constructing multi-signal lateral flow immunoassays (LFIA). In this study, a ZrFe-MOF@PtNPs nanocomposite was synthesized by uniformly depositing platinum nanoparticles (PtNPs) onto the surface of ZrFe-MOFs using an impregnation-reduction method. The ZrFe-MOF@PtNPs exhibited broad absorption spectra, excellent peroxidase-like activity (SA = 21.77 U/mg), high solvent stability, and efficient antibody binding capability. A portable LFIA platform was developed based on ZrFe-MOF@PtNPs and a smartphone for the targeted detection of carcinogenic aflatoxins. This method enabled the readout of colorimetric, fluorescent, and catalytic signals, significantly enhancing detection sensitivity, ensuring result accuracy, and expanding the dynamic detection range. For aflatoxin M1, the calculation of the detection limit of the three signal modes reached as low as 0.0062 ng/mL, which is two orders of magnitude more sensitive than AuNPs-LFIA (0.1839 ng/mL). This study provides effective guidance for multifunctional modification of MOFs and serves as a reference for the application of MOF-based nanozymes in point-of-care biosensors.
开发更灵敏、稳定和便携的生物传感器对于满足多样化和复杂检测环境的需求至关重要。基于 MOF 的纳米酶已成为出色的光学报告器，使其成为构建多信号横向流动免疫分析（LFIA）的理想信号供体。在这项研究中，通过浸渍-还原法将铂纳米粒子（PtNPs）均匀沉积在 ZrFe-MOFs 的表面上，合成了 ZrFe-MOF@PtNPs 纳米复合材料。ZrFe-MOF@PtNPs 具有宽的吸收光谱、优异的过氧化物酶样活性（SA=21.77 U/mg）、高溶剂稳定性和有效的抗体结合能力。基于 ZrFe-MOF@PtNPs 和智能手机开发了一种便携式 LFIA 平台，用于靶向检测致癌黄曲霉毒素。该方法实现了比色、荧光和催化信号的读取，显著提高了检测灵敏度，确保了结果的准确性，并扩展了动态检测范围。对于黄曲霉毒素 M1，三种信号模式的检测限计算低至 0.0062 ng/mL，比 AuNPs-LFIA（0.1839 ng/mL）灵敏两个数量级。本研究为 MOFs 的多功能修饰提供了有效的指导，并为基于 MOF 的纳米酶在即时检测生物传感器中的应用提供了参考。
