Centre for Materials Engineering and Regenerative Medicine, Bharath Institute of Higher Education and Research, Selaiyur, Chennai, Tamil Nadu 600126, India.
School of Chemical Engineering, Yeungnam University, Gyeongsan-si 38541, South Korea; Department of Biotechnology, Saveetha School of Engineering, Saveetha Institute of Medical and Technical Sciences, Chennai 602 105, India.
Ultrason Sonochem. 2024 Dec;111:107069. doi: 10.1016/j.ultsonch.2024.107069. Epub 2024 Sep 15.
Alternative to synthetic dyes containing harmful compounds, dyes derived from natural sources are gaining popularity due to their safer and eco-friendly nature. This study focuses on extracting red dye from Basella alba fruit and optimising the extraction methods, including ultrasonic bath, ultrasonic probe, and direct heating. The extracted dye was then used for dyeing cotton, silk, and leather without needing a mordant. Furthermore, the antibacterial properties of the extracted red dye were evaluated against skin bacteria. The UV-Visible spectrophotometric analysis revealed that the maximum red colour in the methanol extract (λ 270 and λ 542 nm) was achieved at 60 °C for 30 min using the ultrasonic water bath extraction method, followed by the ultrasonic probe and direct heating methods. The FTIR spectra confirmed the presence of flavonoids, betacyanin, and gomphrenin-I in the extracted dye. The ultrasonic dyeing process at 50 °C yielded a K/s value 6.3 for the dyed cotton, silk, and leather without using a mordant. Additionally, the fatness test indicated a high grade of 0.5-1.5 for the ultrasonic dyeing method compared to other dyeing techniques. The extracted dye exhibited significant antibacterial activity against all Pseudomonas sp. after extraction in methanol, with the highest inhibition observed against Pseudomonas sp. with a MIC of 1.56 mg/ml.
替代含有有害化合物的合成染料，天然来源的染料由于其更安全、更环保的特性而越来越受欢迎。本研究专注于从白背叶 Basella alba 果实中提取红色染料，并优化提取方法，包括超声浴、超声探头和直接加热。然后，将提取的染料用于对棉、丝和皮革进行染色，而无需媒染。此外，还评估了提取的红色染料对皮肤细菌的抗菌性能。紫外-可见分光光度分析表明，在甲醇提取物中（λ 270 和 λ 542nm），在 60°C 下使用超声水浴提取法提取 30 分钟可获得最大红色（λ 270 和 λ 542nm），其次是超声探头和直接加热法。FTIR 光谱证实了提取染料中存在类黄酮、甜菜红素和gomphrenin-I。在 50°C 下进行超声染色，在不使用媒染剂的情况下，对棉、丝和皮革的染色 K/s 值为 6.3。此外，肥度测试表明，与其他染色技术相比，超声染色法的等级为 0.5-1.5。在甲醇中提取后，提取的染料对所有铜绿假单胞菌均表现出显著的抗菌活性，对铜绿假单胞菌的抑制作用最高，MIC 为 1.56mg/ml。
