Jiangsu Province Engineering Research Center for Marine Bio-resources Sustainable Utilization, Hohai University, Nanjing 210098, China; Department of Marine Biology, College of Oceanography, Hohai University, Nanjing 210098, China.
Jiangsu Province Engineering Research Center for Marine Bio-resources Sustainable Utilization, Hohai University, Nanjing 210098, China; Department of Marine Biology, College of Oceanography, Hohai University, Nanjing 210098, China.
Fish Shellfish Immunol. 2024 Nov;154:109917. doi: 10.1016/j.fsi.2024.109917. Epub 2024 Sep 21.
Galectins are a family of animal lectins involved in the immune response against pathogens. However, the roles of fish galectins during pathogen infection require comprehensive studies. In the present research, eight different galectin genes from Takifugu obscurus (named ToGalec1-8) were identified and characterized. ToGalec1-8 encoded proteins of 240, 182, 373, 145, 452, 135, 359 and 346 amino acids, respectively. All predicted ToGalec1-8 proteins possessed one or more conserved carbohydrate recognition domains (CRDs). Phylogenetic analysis revealed that ToGalec1-8 were evolutionarily closely related to their counterparts in other selected vertebrates, hinting their genetic relationship. Tissue distribution analysis showed that most ToGalec genes were distributed ubiquitously in all detected tissues, with relatively high expression in immune tissues. After stimulation by Vibrio harveyi and Staphylococcus aureus, the mRNA transcripts of ToGalec1-8 in liver and kidney were significantly upregulated. In addition, RNA interference experiments indicated that knockdown of ToGalec1 and ToGalec7 inhibited the clearance of bacteria in vivo. Taken together, these obtained results suggested that ToGalec1-8 play an important role in innate immunity and defense against bacterial infection in T. obscurus.
半乳糖凝集素是一类参与动物免疫反应抵御病原体的凝集素。然而，鱼类半乳糖凝集素在病原体感染过程中的作用仍需要全面研究。本研究从暗纹东方鲀（Takifugu obscurus）中鉴定并分析了 8 个不同的半乳糖凝集素基因（命名为 ToGalec1-8）。ToGalec1-8 编码的蛋白分别由 240、182、373、145、452、135、359 和 346 个氨基酸组成。所有预测的 ToGalec1-8 蛋白均具有一个或多个保守的碳水化合物识别结构域（CRD）。系统进化分析表明，ToGalec1-8 与其他选择的脊椎动物中的同源物在进化上密切相关，暗示了它们的遗传关系。组织分布分析表明，大多数 ToGalec 基因在所有检测到的组织中广泛分布，在免疫组织中表达较高。在哈维弧菌和金黄色葡萄球菌刺激后，肝和肾组织中 ToGalec1-8 的 mRNA 转录物显著上调。此外，RNA 干扰实验表明，ToGalec1 和 ToGalec7 的敲低抑制了体内细菌的清除。综上所述，这些结果表明，ToGalec1-8 在暗纹东方鲀的固有免疫和抵御细菌感染中发挥重要作用。
