Tsang Benjamin, Gerlai Robert
Department of Cell & Systems Biology, University of Toronto, Toronto, Ontario, Canada.
Department of Psychology, University of Toronto Mississauga, Mississauga, Ontario, L5L 1C6, Canada; Department of Cell & Systems Biology, University of Toronto, Toronto, Ontario, Canada.
Trends Neurosci. 2024 Dec;47(12):985-993. doi: 10.1016/j.tins.2024.08.013. Epub 2024 Sep 21.
Although zebrafish (Danio rerio) neuroscience research is rapidly expanding, the fundamental question of how these fish should be maintained in research laboratories remains largely unstudied. This may explain the diverse practices and broad range of environmental parameters used in zebrafish facilities. Here, we provide examples of these parameters and practices, including housing density, tank size, and water chemistry. We discuss the principles of stochastic resonance versus homeostasis and provide hypothetical examples to explain why keeping zebrafish outside of their tolerated range of environmental parameters may increase phenotypical variance and reduce replicability. We call for systematic studies to establish the optimal maintenance conditions for zebrafish. Furthermore, we discuss why knowing more about the natural behavior and ecology of this species could be a guiding principle for these studies.
尽管斑马鱼（Danio rerio）神经科学研究正在迅速发展，但在研究实验室中应如何饲养这些鱼类这一基本问题在很大程度上仍未得到研究。这或许可以解释斑马鱼养殖设施中存在的各种饲养方式和广泛的环境参数。在此，我们列举了这些参数和饲养方式的示例，包括饲养密度、鱼缸大小和水化学性质。我们讨论了随机共振与稳态的原理，并提供假设示例来解释为何将斑马鱼饲养在其可耐受的环境参数范围之外可能会增加表型变异并降低可重复性。我们呼吁开展系统性研究以确定斑马鱼的最佳饲养条件。此外，我们还讨论了为何更多地了解该物种的自然行为和生态可能是这些研究的指导原则。
