Leist T, Titmas R, Parti S, Meager A
Mol Immunol. 1985 Aug;22(8):929-36. doi: 10.1016/0161-5890(85)90079-3.
Synthetic polypeptides corresponding to hydrophilic regions of human interferon gamma (HuIFN gamma) based on the amino acid sequence of HuIFN gamma inferred from its cDNA sequence were used to produce antibodies in rabbits which reacted with the polypeptides and which might also be expected to recognise native HuIFN gamma. Groups of 3 or 4 rabbits were immunised with synthetic polypeptides corresponding to HuIFN gamma amino acid sequences 1-20, 1-59, 24-59, 36-59 and 87-96 which included major hydrophilic domains of the IFN gamma molecule. All the rabbits produced antibodies which recognised the polypeptide immunogen, but to date only 1 of 4 rabbits immunised with polypeptide 24-59 and 1 of 3 rabbits immunised with polypeptide 1-59 have produced antibodies which also recognise native HuIFN gamma. The positively reacting antiserum from the rabbit immunised with polypeptide 24-59 could only be shown to weakly bind to HuIFN gamma, whereas the positively reacting antiserum from the rabbit immunised with polypeptide 1-59 was shown to both weakly bind to HuIFN gamma and weakly neutralise its in vitro antiviral effect. The results so far obtained suggest that the amino acid sequences close to the N-terminus are important for biological activity.
根据从人干扰素γ（HuIFNγ）cDNA序列推导的氨基酸序列，合成了对应于HuIFNγ亲水区域的多肽，用于在兔体内产生抗体，这些抗体可与多肽发生反应，也有望识别天然HuIFNγ。用对应于HuIFNγ氨基酸序列1 - 20、1 - 59、24 - 59、36 - 59和87 - 96的合成多肽免疫3或4只兔的组，这些序列包含IFNγ分子的主要亲水结构域。所有兔都产生了识别多肽免疫原的抗体，但迄今为止，用多肽24 - 59免疫的4只兔中只有1只，以及用多肽1 - 59免疫的3只兔中只有1只产生了也能识别天然HuIFNγ的抗体。用多肽24 - 59免疫的兔产生的阳性反应抗血清仅显示与HuIFNγ有弱结合，而用多肽1 - 59免疫的兔产生的阳性反应抗血清显示既与HuIFNγ有弱结合，又能弱中和其体外抗病毒作用。目前获得的结果表明，靠近N端的氨基酸序列对生物活性很重要。
