Meier Bürgisser Gabriella, Evrova Olivera, Heuberger Dorothea M, Wolint Petra, Rieber Julia, Miescher Iris, Schüpbach Reto A, Giovanoli Pietro, Calcagni Maurizio, Buschmann Johanna
Division of Plastic Surgery and Hand Surgery, University Hospital Zurich, Sternwartstrasse 14, 8091 Zurich, Switzerland.
Laboratory of Applied Mechanobiology, ETH Zürich, Vladimir-Prelog-Weg 1-5/ 10, 8093 Zurich, Switzerland.
Data Brief. 2024 Sep 2;57:110886. doi: 10.1016/j.dib.2024.110886. eCollection 2024 Dec.
The first set of data refers to PAR-2 gene expression with the target gene rbF2rl1 assessed in tenocytes harvested from New Zealand White Rabbits' Achilles tendons. These tenocytes were stimulated with 20 ng/mL platelet-derived growth factor-BB (PDGF-BB) and compared to the corresponding cell culture without growth factor PDGF-BB. In addition, three inhibitors were tested. In the presence or absence of 40 µM inhibitor concentration and 5 % fetal bovine serum, the following inhibitors were applied: SB203580 = inhibitor for MAPK; LY-294002 = inhibitor for PI3K; PD153035 = inhibitor for EGFR. As control, gene expression was assessed under DMSO = dimethyl sulfoxide (solvent of the inhibitors) or in medium = basal culture medium (with 10 % fetal bovine serum). The second set of data represents morphological aspects of cytoskeletal reorganization for rabbit Achilles tenocytes stimulated with 20 ng/mL PDGF-BB compared to the corresponding cell culture without PDGF-BB. Data on cell size, on F-actin immunohistochemical labeling intensity, α-tubulin immunohistochemical labeling intensity and on cell aspect ratio (length of the cell divided by its width) are presented. Moreover, analogous to the first set of data, cytoskeletal rearrangement in the presence or absence of the inhibitors SB203580, LY-294002 and PD153035 in the presence or absence of PDGF-BB were assessed.
第一组数据涉及在从新西兰白兔跟腱中采集的腱细胞中评估的PAR-2基因表达，其靶基因为rbF2rl1。这些腱细胞用20 ng/mL血小板衍生生长因子-BB（PDGF-BB）刺激，并与没有生长因子PDGF-BB的相应细胞培养物进行比较。此外，测试了三种抑制剂。在存在或不存在40 µM抑制剂浓度和5%胎牛血清的情况下，应用了以下抑制剂：SB203580 = MAPK抑制剂；LY-294002 = PI3K抑制剂；PD153035 = EGFR抑制剂。作为对照，在二甲基亚砜（DMSO，抑制剂的溶剂）或培养基（基础培养基，含10%胎牛血清）中评估基因表达。第二组数据表示用20 ng/mL PDGF-BB刺激的兔跟腱腱细胞与没有PDGF-BB的相应细胞培养物相比，细胞骨架重组的形态学方面。呈现了关于细胞大小、F-肌动蛋白免疫组织化学标记强度、α-微管蛋白免疫组织化学标记强度以及细胞长宽比（细胞长度除以宽度）的数据。此外，与第一组数据类似，评估了在存在或不存在PDGF-BB的情况下，抑制剂SB203580、LY-294002和PD153035存在或不存在时的细胞骨架重排。
