Ofenstein J P, Rillema J A, Lawson D M
Proc Soc Exp Biol Med. 1985 Nov;180(2):236-9. doi: 10.3181/00379727-180-42170.
The mitogenic action of prolactin in Nb 2 node lymphoma cells was inhibited by two drugs which interfere with polyamine biosynthesis. At concentrations of 0.5 mM and above alpha-difluoromethyl ornithine (DFMO), which inhibits ornithine decarboxylase and the conversion of ornithine to putrescine, significantly attenuated the mitogenic effect of prolactin. This inhibition was prevented by the addition of putrescine, spermidine, or spermine to the culture medium. At concentrations of 1 microM and above methylglyoxal bis(guanylhydrazone) (MGBG), which inhibits S-adenosylmethionine decarboxylase and hence the conversion of putrescine to spermidine and spermine, abolished the mitogenic action of prolactin. This inhibition was prevented by the addition of spermidine or spermine, but not putrescine, to the culture medium. These studies show that ongoing polyamine biosynthesis is essential for prolactin to express its mitogenic effect in this lymphoma cell line.
两种干扰多胺生物合成的药物抑制了催乳素在Nb 2 淋巴瘤细胞中的促有丝分裂作用。浓度在0.5 mM及以上时，抑制鸟氨酸脱羧酶以及鸟氨酸向腐胺转化的α-二氟甲基鸟氨酸（DFMO）显著减弱了催乳素的促有丝分裂作用。向培养基中添加腐胺、亚精胺或精胺可防止这种抑制作用。浓度在1 μM及以上时，抑制S-腺苷甲硫氨酸脱羧酶从而抑制腐胺向亚精胺和精胺转化的甲基乙二醛双（脒腙）（MGBG）消除了催乳素的促有丝分裂作用。向培养基中添加亚精胺或精胺可防止这种抑制作用，但添加腐胺则不能。这些研究表明，持续的多胺生物合成对于催乳素在该淋巴瘤细胞系中发挥其促有丝分裂作用至关重要。
