Vaidya Ameya, Bankier Claire, Johnston Helinor, Bridle Helen
Institute of Biological Chemistry, Biophysics and Bioengineering, Heriot Watt University, Edinburgh EH14 4AS, UK.
Methods Protoc. 2024 Aug 23;7(5):66. doi: 10.3390/mps7050066.
The extraction of DNA from oocysts is challenging due to the robust oocyst wall. Nanoparticles have been applied to disinfect oocysts; here, we demonstrate the utilisation of nanoparticles to disrupt the oocyst wall to enable sporozoite lysis and detection via PCR. Both silver and zinc oxide nanoparticles are investigated under different conditions and compared to existing techniques. Zinc oxide nanoparticles are shown to be as effective as freeze-thaw methods, suggesting that a nanoparticle lysis approach offers a viable alternative to existing methods.
由于卵囊壁坚固，从卵囊中提取DNA具有挑战性。纳米颗粒已被用于对卵囊进行消毒；在此，我们展示了利用纳米颗粒破坏卵囊壁，以使子孢子裂解并通过聚合酶链反应（PCR）进行检测。在不同条件下对银纳米颗粒和氧化锌纳米颗粒进行了研究，并与现有技术进行了比较。结果表明，氧化锌纳米颗粒与冻融法一样有效，这表明纳米颗粒裂解方法为现有方法提供了一种可行的替代方案。
