Illinois Zoological and Aquatic Animal Residency, Shedd Aquarium, Chicago, IL 60605, USA; University of Illinois College of Veterinary Medicine, Urbana, IL 61802, USA; Chicago Zoological Society, Brookfield Zoo, Brookfield, IL 60513, USA; Wildlife Epidemiology Laboratory, University of Illinois, Urbana, IL 61802, USA.
Chicago Zoological Society, Brookfield Zoo, Brookfield, IL 60513, USA; Wildlife Epidemiology Laboratory, University of Illinois, Urbana, IL 61802, USA; Veterinary Diagnostic Laboratory, University of Illinois, Urbana, IL 61802, USA.
J Virol Methods. 2024 Dec;330:115033. doi: 10.1016/j.jviromet.2024.115033. Epub 2024 Sep 21.
In 2007, a mortality event involving over 100 Sulawesi tortoises (Indotestudo forsteni), two Impressed tortoises (Manouria impress) and a critically endangered Burmese star tortoise (Geochelone platynota) was attributed to Sulawesi tortoise adenovirus (STADV; genus Siadenovirus). We developed a TaqMan quantitative PCR assay targeting the DNA polymerase gene of STADV for use in clinical diagnosis and epidemiologic surveillance. This assay failed to amplify five closely-related chelonian adenoviruses, indicating high analytical specificity. The assay performed with high efficiency (slope = -3.337; R = 0.999) and high inter- and intra-assay repeatability (coefficient of variation <1.36 % at all standard curve dilutions). Dynamic range included 1.00 × 10 to 1.00 × 10 target copies per reaction and limit of detection was 10 target copies per reaction, though 10 target copies per reaction were intermittently detected. This qPCR assay provides a valuable diagnostic tool for characterization of STADV epidemiology, including potential identification of the North American reservoir host.
2007 年，一起涉及超过 100 只苏拉威西象龟（Indotestudo forsteni）、2 只靴脚陆龟（Manouria impress）和 1 只极度濒危的缅甸星龟（Geochelone platynota）的死亡事件归因于苏拉威西象龟腺病毒（STADV；属 Siadenovirus）。我们开发了一种针对 STADV DNA 聚合酶基因的 TaqMan 定量 PCR 检测方法，用于临床诊断和流行病学监测。该检测方法未能扩增五种密切相关的龟类腺病毒，表明具有很高的分析特异性。该检测方法具有高效性（斜率=-3.337；R=0.999）和高的内、外重复性（在所有标准曲线稀释度下，变异系数<1.36%）。动态范围包括 1.00×10 至 1.00×10 个靶拷贝/反应，检测限为 10 个靶拷贝/反应，但 10 个靶拷贝/反应时有间歇性检测到。该 qPCR 检测方法为 STADV 流行病学特征的鉴定提供了一个有价值的诊断工具，包括对北美储主宿主的潜在鉴定。
