Leitch G J
Alcohol Alcohol. 1985;20(3):305-11.
The submandibular gland complex was removed from young adult Wistar rats. Sham operations were performed on control animals. Three weeks later, the animals were starved for 24 hr and after pyloric ligation the stomachs were instilled with 30% ethanol. Two hours later, the animals were killed and the surface areas of the ulcerated mucosae were measured and the values used as ulcer index scores. In male, but not female, rats prior sialoadenectomy significantly exacerbated ethanol-induced gastric ulceration of the glandular stomach mucosa. This increase in ulceration was accompanied by an increase in the backflux of acid into the injured tissue. Sialoadenectomy had no statistically significant effect on histamine-stimulated gastric acid and pepsin secretion when these were measured 3 weeks later. Epidermal growth factor (EGF) was administered subcutaneously to intact animals in which the stomachs had been filled with 30% ethanol. When administered at a dose of 10 micrograms/kg per hr. EGF significantly reduced the ethanol-induced ulceration. High molecular weight nerve growth factor (7S NGF) was administered intragastrically prior to instilling ethanol in the stomachs of intact rats. The NGF had no effect on the severity of ethanol-induced ulcers. These data suggest that saliva protects the gastric mucosa from ethanol-induced ulceration and that salivary EGF is a possible candidate for this protection.
从年轻成年Wistar大鼠身上摘除下颌下腺复合体。对对照动物进行假手术。三周后，将动物禁食24小时，幽门结扎后向胃内注入30%乙醇。两小时后，处死动物，测量溃疡黏膜的表面积，并将这些值用作溃疡指数评分。在雄性大鼠而非雌性大鼠中，术前唾液腺切除术显著加剧了乙醇诱导的腺胃黏膜胃溃疡。溃疡的增加伴随着胃酸反流至受损组织的增加。三周后测量时，唾液腺切除术对组胺刺激的胃酸和胃蛋白酶分泌没有统计学上的显著影响。将表皮生长因子（EGF）皮下注射到胃内已充满30%乙醇的完整动物体内。当以每小时10微克/千克的剂量给药时，EGF显著减轻了乙醇诱导的溃疡。在向完整大鼠胃内注入乙醇之前，将高分子量神经生长因子（7S NGF）经胃给药。NGF对乙醇诱导溃疡的严重程度没有影响。这些数据表明，唾液可保护胃黏膜免受乙醇诱导的溃疡，并且唾液EGF可能是这种保护作用的一个候选因素。
