Institute of Bio- and Geosciences, Forschungszentrum Jülich, Jülich, Germany.
Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures, Braunschweig, Germany.
Microbiol Spectr. 2024 Nov 5;12(11):e0066324. doi: 10.1128/spectrum.00663-24. Epub 2024 Sep 25.
, a multifaceted genus of soil-dwelling bacteria belonging to the phylum , features intricate phage-host interactions shaped by its complex life cycle and the synthesis of a diverse array of specialized metabolites. Here, we describe the isolation and characterization of four novel phages infecting a variety of different host species. While phage Kamino, isolated on , is predicted to be temperate and encodes a serine integrase in its genome, phages Geonosis (isolated on ) and Abafar and Scarif, isolated on , are virulent phages. Phages Kamino and Geonosis were shown to amplify well in liquid culture leading to a pronounced culture collapse already at low titers. Determination of the host range by testing >40 different species identified phages Kamino, Abafar, and Scarif as broad host-range phages. Overall, the phages described in this study expand the publicly available portfolio of phages infecting and will be instrumental in advancing the mechanistic understanding of the intricate antiviral strategies employed by these multicellular bacteria.IMPORTANCEThe actinobacterial genus is characterized by multicellular, filamentous growth and the synthesis of a diverse range of bioactive molecules. These characteristics also play a role in shaping their interactions with the most abundant predator in the environment, bacteriophages-viruses infecting bacteria. In this study, we characterize four new phages infecting . Out of those, three phages feature a broad host range infecting up to 15 different species. The isolated phages were characterized with respect to plaque and virion morphology, host range, and amplification in liquid culture. In summary, the phages reported in this study contribute to the broader collection of publicly available phages infecting , playing a crucial role in advancing our mechanistic understanding of phage-host interactions of these multicellular bacteria.
，一个多方面的土壤细菌属，属于门，其特征是噬菌体 - 宿主相互作用复杂，这是由其复杂的生命周期和多种特殊代谢物的合成所塑造的。在这里，我们描述了四种新型噬菌体的分离和特性，这些噬菌体感染了各种不同的宿主物种。虽然噬菌体 Kamino 是在 上分离出来的，预计是温和的，并且在其基因组中编码丝氨酸整合酶，但噬菌体 Geonosis（在 上分离出来的）和 Abafar 和 Scarif，是在 上分离出来的烈性噬菌体。噬菌体 Kamino 和 Geonosis 在液体培养中表现出良好的扩增，导致在低滴度时就会出现明显的培养物崩溃。通过测试 >40 种不同的 种来确定宿主范围，鉴定出噬菌体 Kamino、Abafar 和 Scarif 是广谱宿主范围的噬菌体。总的来说，本研究中描述的噬菌体扩展了可用于感染的噬菌体的公开组合，并将有助于推进对这些多细胞细菌所采用的复杂抗病毒策略的机制理解。
重要性：放线菌属的特征是多细胞、丝状生长和多种生物活性分子的合成。这些特征也在塑造它们与环境中最丰富的捕食者噬菌体 - 感染细菌的病毒的相互作用中发挥作用。在这项研究中，我们对感染的四种新噬菌体进行了特征描述。其中三种噬菌体具有广谱宿主范围，可以感染多达 15 种不同的物种。分离出的噬菌体在菌斑和病毒形态、宿主范围以及在液体培养中的扩增方面进行了特征描述。总之，本研究中报道的噬菌体丰富了可用于感染的噬菌体的广泛收集，对推进我们对这些多细胞细菌的噬菌体 - 宿主相互作用的机制理解起着至关重要的作用。
