Emerg Infect Dis. 2024 Oct;30(10):1998-2005. doi: 10.3201/eid3010.231509.
In September 2021, eight campylobacteriosis cases were identified in a town in Nebraska, USA. We assessed potential exposures for a case-control analysis. We conducted whole-genome sequencing on Campylobacter isolates from patients' stool specimens. We collected large-volume dead-end ultrafiltration water samples for Campylobacter and microbial source tracking testing at the Centers for Disease Control and Prevention. We identified 64 cases in 2 waves of illnesses. Untreated municipal tap water consumption was strongly associated with illness (wave 1 odds ratio 15.36; wave 2 odds ratio 16.11). Whole-genome sequencing of 12 isolates identified 2 distinct Campylobacter jejuni subtypes (1 subtype/wave). The town began water chlorination, after which water testing detected coliforms. One dead-end ultrafiltration sample yielded nonculturable Campylobacter and avian-specific fecal rRNA genomic material. Our investigation implicated contaminated, untreated, municipal water as the source. Results of microbial source tracking supported mitigation with continued water chlorination. No further campylobacteriosis cases attributable to water were reported.
2021 年 9 月，美国内布拉斯加州的一个城镇发现了 8 例弯曲菌病病例。我们评估了病例对照分析的潜在暴露情况。我们对患者粪便标本中的弯曲菌分离株进行了全基因组测序。我们在美国疾病控制与预防中心收集了大量的死端超滤水样，用于弯曲菌和微生物源追踪检测。我们在 2 波疾病中发现了 64 例病例。未处理的市政自来水消费与疾病强烈相关（第 1 波优势比 15.36；第 2 波优势比 16.11）。对 12 个分离株的全基因组测序鉴定出 2 种不同的空肠弯曲菌亚型（1 种亚型/波）。该镇开始进行水氯化消毒，之后水检测发现了大肠菌群。一个死端超滤水样中含有不可培养的弯曲菌和禽特异性粪便 rRNA 基因组物质。我们的调查表明，污染的、未经处理的市政用水是源头。微生物源追踪的结果支持持续氯化消毒的缓解措施。没有报告与水有关的进一步弯曲菌病病例。
