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犬左心室浦肯野纤维的分布特征。

Distribution characteristics of Purkinje fibres in the canine left ventricle.

机构信息

Department of Cardiology, The General Hospital of Northern Theater Command, Shenyang, Liaoning, China.

Graduate School of China Medical University, China Medical University, Shenyang, Liaoning, China.

出版信息

J Cell Mol Med. 2024 Sep;28(18):e70117. doi: 10.1111/jcmm.70117.

Abstract

Purkinje-related ventricular arrhythmias have been increasingly reported, and with the development of catheter ablation techniques, intervention for Purkinje-related arrhythmias has been shown to be effective. The characteristics of Purkinje fibres orientation in the 12 canine left ventricles were observed at a gross level by staining the endocardium with Lugol's solution. Purkinje fibres were observed microscopically by HE, Masson's, PAS glycogen, and Cx40 immunohistochemical staining. Staining was successful, and the transverse orientation characteristics of Purkinje fibres were observed by Lugol's staining, and the longitudinal distribution was observed microscopically. The distribution of Purkinje fibres in the canine left ventricle is 'graded', 'layered', and 'networked', which can guide catheter ablation of Purkinje-related ventricular arrhythmia.

摘要

浦肯野相关室性心律失常的报道日益增多,随着导管消融技术的发展,浦肯野相关心律失常的干预已被证明是有效的。通过用卢戈氏液染色心内膜,在大体水平上观察 12 只犬左心室浦肯野纤维的方向特征。用 HE、Masson、PAS 糖原和 Cx40 免疫组化染色观察浦肯野纤维的显微镜特征。染色成功,卢戈氏染色观察到浦肯野纤维的横向取向特征,显微镜下观察到纵向分布。犬左心室浦肯野纤维的分布呈“分级”、“分层”和“网络化”,可指导浦肯野相关室性心律失常的导管消融。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ef69/11423647/bb7f6f301c0b/JCMM-28-e70117-g002.jpg

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