Li WenWen, Liu FeiFei, Chen ShuaiFei, Wingfield Michael J, Duong Tuan A
Department of Plant and Soil Sciences, Forestry and Agricultural Biotechnology Institute (FABI), University of Pretoria, Pretoria 0028, South Africa.
Research Institute of Fast-growing Trees (RIFT), Chinese Academy of Forestry (CAF), ZhanJiang 524022, GuangDong Province, China.
Phytopathology. 2025 Jan;115(1):97-105. doi: 10.1094/PHYTO-05-24-0154-R. Epub 2025 Jan 17.
causes a serious and widespread disease known as Calonectria leaf blight in plantations of southern China. Little is known regarding the population biology or reproductive biology of this pathogen in the affected areas. The aims of this study were to investigate the genetic diversity, population structure, and reproductive mode of . from affected plantations of southern China. Ten polymorphic simple sequence repeat markers were developed for the species and were used to genotype 311 isolates from eight populations. The mating types of all isolates were identified using the gene primers. The results revealed a high level of genetic diversity of the pathogen in all investigated populations. Of the 90 multilocus genotypes detected, 10 were shared between at least two populations. With the exception of one population from HuiZhou, GuangDong (7HZ), the most dominant genotype was shared in the seven remaining populations. Discriminant analysis of principal components and population differentiation analyses showed that the 7HZ population was well differentiated from the others and that there was no significant differentiation between the remaining populations. Analysis of molecular variance suggested that most molecular variation was within populations (86%). Index of association analysis was consistent with a predominantly asexual life cycle for . in the studied regions. Although both mating types were detected in seven of the eight populations, the / ratios in these populations deviated significantly from the 1:1 ratio expected in a randomly mating population.
在中国南方种植园中引发一种名为拟罗耳病叶枯病的严重且广泛传播的病害。关于该病原菌在受影响地区的种群生物学或繁殖生物学知之甚少。本研究的目的是调查来自中国南方受影响种植园的[病原菌名称未给出]的遗传多样性、种群结构和繁殖模式。为该物种开发了10个多态性简单序列重复标记，并用于对来自8个种群的311个分离株进行基因分型。使用[具体基因名称未给出]基因引物鉴定所有分离株的交配型。结果显示，在所有调查种群中该病原菌具有高度的遗传多样性。在所检测到的90种多位点基因型中，有10种在至少两个种群中共享。除了来自广东惠州的一个种群（7HZ）外，最主要的基因型在其余7个种群中共享。主成分判别分析和种群分化分析表明，7HZ种群与其他种群有明显分化，而其余种群之间没有显著分化。分子方差分析表明，大多数分子变异存在于种群内（86%）。关联指数分析与所研究地区的[病原菌名称未给出]主要为无性生命周期一致。尽管在8个种群中的7个种群中都检测到了两种交配型，但这些种群中的[交配型具体表示未给出]比例与随机交配种群预期的1:1比例有显著偏差。
