Plant Physiology, Institute of Plant Biology and Biotechnology, University of Muenster, Muenster, Germany.
Université Paris-Saclay, CEA, CNRS, Institute for Integrative Biology of the Cell (I2BC), Gif-sur-Yvette, France.
Mol Cell Proteomics. 2024 Nov;23(11):100845. doi: 10.1016/j.mcpro.2024.100845. Epub 2024 Sep 24.
Protein acetylation is a key co- and post-translational modification. However, how different types of acetylation respond to environmental stress is still unknown. To address this, we investigated the role of a member of the newly discovered family of plastid acetyltransferases (GNAT2), which features both lysine- and N-terminal acetyltransferase activities. Our study aimed to provide a holistic multi-omics acetylation-dependent view of plant acclimation to short-term light changes. We found that both the yield and coverage of the N-terminal acetylome remained unchanged in WT and gnat2-KO backgrounds after 2 h of exposure to high light or darkness. Similarly, no differences in transcriptome or adenylate energy charge were observed between the genotypes under the tested light conditions. In contrast, the lysine acetylome proved to be sensitive to the changes in light conditions, especially in the gnat2 background. This suggests unique strategies of plant acclimation for quick responses to environmental changes involving lysine, but not N-terminal, GNAT2-mediated acetylation activity.
蛋白质乙酰化是一种关键的共翻译后修饰。然而，不同类型的乙酰化如何响应环境压力仍然未知。为了解决这个问题，我们研究了一个新发现的质体乙酰转移酶（GNAT2）家族成员的作用，该家族成员具有赖氨酸和 N 端乙酰转移酶活性。我们的研究旨在提供一个整体的多组学乙酰化依赖的植物对短期光变化的适应观点。我们发现，在高光照或黑暗暴露 2 小时后，WT 和 gnat2-KO 背景下的 N 端乙酰组的产量和覆盖度保持不变。同样，在测试的光照条件下，两种基因型之间的转录组或腺苷酸能量电荷没有差异。相比之下，赖氨酸乙酰组被证明对光照条件的变化很敏感，尤其是在 gnat2 背景下。这表明植物适应环境变化的独特策略涉及赖氨酸，但不涉及 N 端，由 GNAT2 介导的乙酰化活性。
