保卫细胞过氧化氢抗性 1 蛋白在拟南芥保卫细胞脱落酸信号中位于活性羰基产物产生的上游。
GUARD CELL HYDROGEN PEROXIDE-RESISTANT1 functions upstream of reactive carbonyl species production in Arabidopsis guard-cell abscisic acid signaling.
机构信息
Graduate School of Environmental and Life Science, Okayama University, Okayama, Japan.
School of Agriculture, Okayama University, Okayama, Japan.
出版信息
Biosci Biotechnol Biochem. 2024 Nov 22;88(12):1403-1410. doi: 10.1093/bbb/zbae135.
GUARD CELL HYDROGEN PEROXIDE-RESISTANT1 (GHR1), a leucine-rich repeat receptor-like kinase, is involved in abscisic acid (ABA)-induced stomatal closure. We investigated the role of GHR1 in reactive oxygen species (ROS) signaling for ABA-induced stomatal closure. ABA induced ROS production in wild type (WT) and the ghr1 of Arabidopsis thaliana. Hydrogen peroxide induced stomatal closure, accompanying the generation of acrolein in guard cells. The reactive carbonyl species (RCS) scavengers inhibited the ABA- and H2O2-induced stomatal closure in WT. In the ghr1, H2O2 failed to induce acrolein production and stomatal closure while RCS induced stomatal closure. Thus, GHR1 functions downstream of ROS and is required for the generation of RCS in guard-cell ABA signaling. In the ghr1, Ca2+ induced stomatal closure but RCS did not activate ICa channels. The GHR1 may be also involved in a Ca2+-independent pathway for ABA-induced stomatal closure.
保卫细胞过氧化氢抗性 1（GHR1），一种富含亮氨酸重复受体样激酶，参与脱落酸（ABA）诱导的气孔关闭。我们研究了 GHR1 在 ABA 诱导的气孔关闭的活性氧（ROS）信号传导中的作用。ABA 诱导野生型（WT）和拟南芥 ghr1 的 ROS 产生。过氧化氢诱导保卫细胞中丙烯醛的产生，引起气孔关闭。活性羰基物质（RCS）清除剂抑制 WT 中 ABA 和 H2O2 诱导的气孔关闭。在 ghr1 中，H2O2 未能诱导丙烯醛的产生和气孔关闭，而 RCS 诱导气孔关闭。因此，GHR1 在 ROS 下游起作用，并在保卫细胞 ABA 信号传导中产生 RCS 。在 ghr1 中，Ca2+诱导气孔关闭，但 RCS 不会激活 ICa 通道。GHR1 可能也参与 ABA 诱导的气孔关闭的 Ca2+-独立途径。
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