Chukwu C C
Int J Zoonoses. 1985 Mar;12(1):6-13.
The diagnostic efficiency of the Brucellin skin test for bovine brucellosis was evaluated using cattle of known history. The test was negative in six out of 14 heifers (42.9%) infected with virulent Brucella abortus (Br. abortus) strain 544. In four cattle vaccinated with a reduced dose of Br. abortus strain 19 vaccine (5 X 10(9) live organisms) the skin test became positive in all the animals but two weeks after immunization. However, all the vaccinates became negative 14 weeks after vaccination, whereas nine out of 14 heifers (64.3%) vaccinated with killed Br. abortus 45/20 adjuvant vaccine (Duphavac vaccine) were still positive 18 months post immunization. Four control cattle were persistently negative. It was considered that the procedure would be most useful for testing non-exposed cattle and should be used as a screen test. It is not useful as a diagnostic test for cattle immunized with Duphavac vaccine. A recommendation is made for interpreting the Brucellin skin test for the diagnosis of bovine brucellosis in non-exposed herds.
使用已知病史的牛群评估布鲁菌素皮肤试验对牛布鲁氏菌病的诊断效率。在14头感染强毒流产布鲁氏菌（Br. abortus）544株的小母牛中，有6头（42.9%）的试验结果为阴性。在4头接种了减毒布鲁氏菌19号疫苗（5×10⁹个活菌体）的牛中，皮肤试验在免疫后两周时所有动物均呈阳性。然而，所有接种疫苗的动物在接种后14周均转为阴性，而在14头接种了灭活布鲁氏菌45/20佐剂疫苗（Duphavac疫苗）的小母牛中，有9头（64.3%）在免疫后18个月仍呈阳性。4头对照牛一直呈阴性。认为该方法对检测未接触过布鲁氏菌的牛最为有用，应作为一种筛查试验。对于接种Duphavac疫苗的牛，它作为诊断试验并无用处。针对在未接触过布鲁氏菌的牛群中诊断牛布鲁氏菌病时布鲁菌素皮肤试验的解读提出了一项建议。
