Li Ruobing, Tang Yaqi, Wang Qiao, Zhao Bingjie, Su Wenwen, Wang Baotong, Li Qiang
State Key Laboratory of Crop Stress Resistance and High-Efficiency Production, College of Plant Protection, Northwest A&F University, Yangling, Shaanxi, China.
Plant Cell Environ. 2025 Jan;48(1):711-727. doi: 10.1111/pce.15172. Epub 2024 Sep 25.
Powdery mildew and stripe rust are major diseases on wheat worldwide that cause significant reductions in wheat production. The ribosomal silencing factor (RsfS) has been proven to regulate protein biosynthesis by inhibiting the translation process in bacterial response to stress. However, the role of RsfS in plant resistance to biotic stresses remains unclear. In this study, the RsfS homolog, TaRsfS was isolated from wheat. Overexpression of TaRsfS (TaRsfS-OE) reduces wheat resistance to powdery mildew and stripe rust and TaRsfS knockout (TaRsfS-KO) increases wheat resistance to both diseases without affecting key agronomic traits. The interaction protein of TaRsfS, 12-oxo-phytodienoic acid reductase 1 (TaOPR1), a key enzyme in the biosynthesis of jasmonic acid (JA), was screened and identified. Knocking-down and overexpression of TaOPR1 indicated that TaOPR1 positively regulates wheat resistance to powdery mildew and stripe rust. TaRsfS may regulate TaOPR1 at upstream, bind to the enzyme activity pocket of TaOPR1 and affect TaOPR1 enzyme activity, resulting in a reduced JA biosynthesis and wheat susceptible to powdery mildew and stripe rust. Collectively, TaRsfS is a susceptibility gene and negatively regulates wheat resistance to powdery mildew and stripe rust, and it has good potential for improving wheat resistance by genetic modifications.
白粉病和条锈病是全球小麦的主要病害，会导致小麦产量大幅下降。核糖体沉默因子（RsfS）已被证明在细菌应对压力时通过抑制翻译过程来调节蛋白质生物合成。然而，RsfS在植物对生物胁迫的抗性中的作用仍不清楚。在本研究中，从小麦中分离出RsfS同源物TaRsfS。TaRsfS过表达（TaRsfS-OE）降低了小麦对白粉病和条锈病的抗性，而TaRsfS敲除（TaRsfS-KO）则增强了小麦对这两种病害的抗性，且不影响关键农艺性状。筛选并鉴定了TaRsfS的相互作用蛋白12-氧代-植物二烯酸还原酶1（TaOPR1），它是茉莉酸（JA）生物合成中的关键酶。TaOPR1的敲低和过表达表明TaOPR1正向调节小麦对白粉病和条锈病的抗性。TaRsfS可能在上游调节TaOPR1，与TaOPR1的酶活性口袋结合并影响TaOPR1的酶活性，导致JA生物合成减少，使小麦对白粉病和条锈病敏感。总体而言，TaRsfS是一个感病基因，负向调节小麦对白粉病和条锈病的抗性，通过基因改造提高小麦抗性具有良好潜力。
