Dhundale A R, Furuichi T, Inouye S, Inouye M
J Bacteriol. 1985 Nov;164(2):914-7. doi: 10.1128/jb.164.2.914-917.1985.
Multicopy single-stranded DNA (msDNA) is a short single-stranded linear DNA originally discovered in Myxococcus xanthus and subsequently found in Stigmatella aurantiaca. It exists at an estimated 500 to 700 copies per chromosome (T. Yee, T. Furuichi, S. Inouye, and M. Inouye, Cell 38:203-209, 1984). We found msDNA in other myxobacteria, including Myxococcus coralloides, Cystobacter violaceus, Cystobacter ferrugineus (Cbfe17), Nannocystis exedens, and nine independently isolated strains of M. xanthus. The presence of msDNA in N. exedens would extend its phylogenetic distribution into another family of myxobacteria. Flexibacter elegans, a Cytophaga-like gliding bacteria which may be even more distantly related, also contained an msDNA but at a much lower copy number. msDNA was not detected in closely related strains of the myxobacteria Cystobacter fuscus and C. ferrugineus (Cbfe16 and Cbfe18) and the more distantly related eubacteria Herpetosiphon giganteus, Taxeobacter ocellatus, Lysobacter antibioticus, Lysobacter enzymogenes, Cytophaga johnsonae, Rhodopseudomonas sphaeroides, and Rhodospirillum rubrum. Thus far, msDNA has been found in certain gliding bacteria but not in others.
多拷贝单链DNA（msDNA）是一种短单链线性DNA，最初在黄色粘球菌中发现，随后在橙色标桩菌中发现。据估计，每条染色体存在500至700个拷贝（T. Yee、T. Furuichi、S. Inouye和M. Inouye，《细胞》38:203 - 209，1984年）。我们在其他粘细菌中发现了msDNA，包括珊瑚状粘球菌、紫色囊杆菌、铁锈色囊杆菌（Cbfe17）、伸展纳氏菌以及9个独立分离的黄色粘球菌菌株。伸展纳氏菌中msDNA的存在将其系统发育分布扩展到了粘细菌的另一个家族。秀丽屈挠杆菌是一种类似纤维粘细菌的滑行细菌，可能亲缘关系更远，它也含有msDNA，但拷贝数要低得多。在粘细菌深褐囊杆菌和铁锈色囊杆菌（Cbfe16和Cbfe18）以及亲缘关系更远的真细菌巨大吸管菌、眼状类杆菌、抗生溶杆菌、产酶溶杆菌、约翰逊纤维粘细菌、球形红假单胞菌和深红红螺菌的近缘菌株中未检测到msDNA。到目前为止，msDNA已在某些滑行细菌中发现，但在其他细菌中未发现。
