谷氨酰胺合成酶在肝脏缺血再灌注损伤中的表达动态：对治疗干预的意义。
Dynamics of glutamine synthetase expression in hepatic ischemia-reperfusion injury: Implications for therapeutic interventions.
作者信息
Huang Zhi-Hao, Dong Meng-Qi, Liu Feng-Yong, Zhou Wei-Jie
机构信息
State Key Laboratory of Organ Failure Research, Department of General Surgery, Guangdong Provincial Key Laboratory of Precision Medicine for Gastrointestinal Tumor, Nanfang Hospital, Southern Medical University, Guangzhou 510515, Guangdong Province, China.
Department of Interventional Radiology, Senior Department of Oncology, Fifth Medical Center of PLA General Hospital, Beijing 100853, China.
出版信息
World J Hepatol. 2024 Aug 27;16(8):1177-1184. doi: 10.4254/wjh.v16.i8.1177.
BACKGROUND
Hepatic ischemia-reperfusion injury (IRI) poses a great challenge in liver surgery and transplantation because of oxidative stress and inflammatory responses. The changes in glutamine synthetase (GS) expression during hepatic IRI remain unclear.
AIM
To investigate the dynamic expression of GS during hepatic IRI.
METHODS
Following hepatic ischemia for 1 h and reperfusion, liver tissue samples were collected at 0.5, 6, and 24 hours postreperfusion for fixation, embedding, sectioning. Hematoxylin and eosin staining and GS staining were performed.
RESULTS
GS expression rapidly decreases in hepatocytes around the central vein after IRI, reaching its lowest point at 6 hours postreperfusion, and then gradually recovers.
CONCLUSION
GS is highly sensitive to IRI, highlighting its potential role as an indicator of liver injury states and a target for therapeutic intervention.
背景
由于氧化应激和炎症反应，肝缺血再灌注损伤（IRI）在肝脏手术和移植中构成了巨大挑战。肝IRI期间谷氨酰胺合成酶（GS）表达的变化仍不清楚。
目的
研究肝IRI期间GS的动态表达。
方法
肝脏缺血1小时后再灌注，在再灌注后0.5、6和24小时收集肝组织样本进行固定、包埋、切片。进行苏木精-伊红染色和GS染色。
结果
IRI后中央静脉周围的肝细胞中GS表达迅速降低，在再灌注后6小时达到最低点，然后逐渐恢复。
结论
GS对IRI高度敏感，突出了其作为肝损伤状态指标和治疗干预靶点的潜在作用。
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