Leung A, Rubinstein S, Yang C, Li J W, Leighton T
Mol Gen Genet. 1985;201(1):96-8. doi: 10.1007/BF00397992.
Mutations (crsA47 and crsA4) in the major sigma factor gene (rpoD) of Bacillus subtilis RNA polymerase have been found to be powerful intergenic suppressors of spoOB, spoOE, spoOF, spoOK and spoIIG mutations. The crsA47 suppressor restores sporulation of spoOE, spoOF, spoOK and spoIIG mutants to levels near those of wild type bacteria and substantially improves the sporulation of a spoOB strain. The crsA mutations are shown to prevent the induction by aliphatic alcohols of SpoO phenocopies in wild type B. subtilis cells.
已发现枯草芽孢杆菌RNA聚合酶的主要σ因子基因（rpoD）中的突变（crsA47和crsA4）是spoOB、spoOE、spoOF、spoOK和spoIIG突变的强大基因间抑制子。crsA47抑制子可将spoOE、spoOF、spoOK和spoIIG突变体的芽孢形成恢复到接近野生型细菌的水平，并显著改善spoOB菌株的芽孢形成。结果表明，crsA突变可阻止野生型枯草芽孢杆菌细胞中脂肪醇诱导SpoO拟表型。
