在鼠伤寒沙门氏菌中，氰化钠被激活成为一种有毒但无致突变性的代谢产物。
Activation of sodium cyanide to a toxic but non-mutagenic metabolite in Salmonella typhimurium.
作者信息
Owais W M, Janakat S, Hunaiti A
出版信息
Mutat Res. 1985 Nov;144(3):119-25. doi: 10.1016/0165-7992(85)90126-5.
Salmonella typhimurium strains (OASS-positive) synthesize a toxic but non-mutagenic metabolite from cyanide and O-acetylserine. Salmonella typhimurium mutant DW379 (OASS-deficient) is neither able to carry out this reaction in vitro nor produce the toxic metabolite in vivo. L-Cysteine reverses the cyanide metabolite mediated inhibition and thus allows OASS-positive strains to grow in medium containing cyanide and O-acetylserine. The results suggest that the enzyme O-acetylserine sulfhydrylase catalyzes the reaction of cyanide and O-acetylserine to form the toxic metabolite. This metabolite is ninhydrin-positive, adheres strongly to the cation-exchange column, and migrates in TLC to an Rf value similar to that of beta-cyanoalanine.
鼠伤寒沙门氏菌菌株（OASS阳性）可从氰化物和O-乙酰丝氨酸合成一种有毒但无致突变性的代谢物。鼠伤寒沙门氏菌突变体DW379（OASS缺陷型）既不能在体外进行此反应，也不能在体内产生有毒代谢物。L-半胱氨酸可逆转氰化物代谢物介导的抑制作用，从而使OASS阳性菌株能够在含有氰化物和O-乙酰丝氨酸的培养基中生长。结果表明，O-乙酰丝氨酸巯基酶催化氰化物与O-乙酰丝氨酸反应形成有毒代谢物。这种代谢物茚三酮呈阳性，与阳离子交换柱结合紧密，在薄层层析中的迁移Rf值与β-氰基丙氨酸相似。
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