Owais W M, Kleinhofs A, Nilan R A
Mutat Res. 1981 Dec;84(2):239-46. doi: 10.1016/0027-5107(81)90193-7.
Cell-free extracts of Salmonella typhimurium synthesize a mutagenic azide metabolite from sodium azide and O-acetylserine. S. typhimurium mutant DW379 (O-acetylserine sulfhydrylase-deficient) extracts were neither able to carry out this reaction not produce the mutagenic azide metabolite in vivo. The in vitro reaction was inhibited by sulfide but not by L-cysteine. The catalytic activity responsible for the mutagenic metabolite synthesis was stable to brief heating up to 55 degrees C and had a pH optimum between 7-7.4. These results suggest that the enzyme O-acetylserine sulfhydrylase catalyzes the reaction of azide with O-acetylserine to form a mutagenic azide metabolite.
鼠伤寒沙门氏菌的无细胞提取物能从叠氮化钠和O-乙酰丝氨酸合成一种诱变叠氮代谢物。鼠伤寒沙门氏菌突变体DW379（缺乏O-乙酰丝氨酸巯基酶）的提取物既不能进行此反应，也不能在体内产生诱变叠氮代谢物。体外反应受到硫化物的抑制，但不受L-半胱氨酸的抑制。负责诱变代谢物合成的催化活性在高达55摄氏度的短暂加热下稳定，最适pH值在7 - 7.4之间。这些结果表明，O-乙酰丝氨酸巯基酶催化叠氮化物与O-乙酰丝氨酸反应形成诱变叠氮代谢物。
