Relationship of Typhimurium 14028 strain and its and mutants with gut microbiota dysbiosis in rats.

Disruptions in gut microbiota, known as dysbiosis, have been increasingly linked to pathogenic infections, with Typhimurium being a notable contributor to these disturbances. We hypothesize that the . Typhimurium 14028 WT strain induces significant dysbiosis in the rat gut microbiota and that the and genes play crucial roles in this process.. In this study, it was aimed at investigating the dysbiotic activity of the . Typhimurium 14028 WT strain on the rat gut microbiota and the roles of and genes on this activity. Changes in the rat gut microbiota were determined by examining the anal swap samples taken from the experimental groups of these animals using 16S rRNA high-throughput sequencing technology. In the experimental groups, the dominant phyla were determined to be and (<0.05). However, while the rate of was significantly reduced in those treated with the WT and mutants, no significant difference was observed in the mutant compared to the control group (<0.05). In all experimental animals, the dominant species was determined to be , regardless of the experiment time and application. The analysis results of the samples taken on the third day from the rat groups infected with the Typhimurium 14028 WT strain (W2) presented the most striking data of this study. Through distance analysis, we demonstrated that a successful infection completely changes the composition of the microbiota, dramatically reduces species diversity and richness in the microbiota and encourages the growth of opportunistic pathogens.