Department of Stomatology Centre, The First People's Hospital of Yunnan Province/Affiliated Hospital of Kunming University of Science and Technology, Kunmming, Yunnan, China.
Folia Histochem Cytobiol. 2024;62(3):133-144. doi: 10.5603/fhc.101230. Epub 2024 Sep 27.
Periodontitis is a serious gum infection that disrupts the soft tissue around teeth. This study aimed to identify the most effective fraction of the Chinese medicine Kangfuxin for periodontitis treatment in a rat model.
Kangfuxin solution was subjected to sequential extraction using chloroform, ethyl acetate, n-butanol, and water. The extracts were evaporated, dissolved in DMSO, diluted in water, and administered to rats via gavage (0.5 mL/day) for 2 weeks. The n-butanol extract was further fractionated using macroporous resin chromatography with 10%, 30%, 50%, 70%, and 90% ethanol elution. Levels of inflammatory cytokines IL-6, IL-1β, and TNF-α in periodontitis samples were examined by ELISA. Leukocyte infiltration in the cementum was analysed by haematoxylin and eosin (H&E) staining.
The n-butanol extract showed the best anti-inflammatory effect, reducing IL-6, IL-1β, and TNF-α levels in periodontitis samples and alleviating tissue damage and leukocyte infiltration in the cementum. Further fractionation revealed that the 50% ethanol fraction of the n-butanol extract had the most potent action in attenuating inflammation. This fraction suppressed the activation of the PI3K-AKT-mTOR signalling pathway in periodontitis samples. Application of a PI3K activator counteracted the anti-inflammatory effect of the 50% ethanol fraction.
We identified a potent anti-inflammatory fraction (50% ethanol fraction of the n-butanol extract) of Kangfuxin for periodontitis treatment. This fraction suppressed the activity of the PI3K-AKT-mTOR signalling pathway in periodontitis samples. Further research is needed to isolate and characterise the specific bioactive compounds within this fraction.
牙周炎是一种严重的牙龈感染，会破坏牙齿周围的软组织。本研究旨在确定中药康复新液治疗牙周炎的最有效部位。
采用氯仿、乙酸乙酯、正丁醇和水对康复新液进行连续提取。提取液蒸发，溶于 DMSO，稀释于水中，通过灌胃（每天 0.5 毫升）给大鼠给药 2 周。正丁醇提取物进一步用大孔树脂层析，用 10%、30%、50%、70%和 90%乙醇洗脱。通过 ELISA 检测牙周炎样本中炎症细胞因子 IL-6、IL-1β和 TNF-α的水平。通过苏木精和伊红（H&E）染色分析牙骨质中的白细胞浸润。
正丁醇提取物显示出最好的抗炎效果，降低牙周炎样本中 IL-6、IL-1β和 TNF-α的水平，并减轻牙骨质的组织损伤和白细胞浸润。进一步的分离表明，正丁醇提取物的 50%乙醇级分在减轻炎症方面作用最强。该级分抑制了牙周炎样本中 PI3K-AKT-mTOR 信号通路的激活。应用 PI3K 激活剂拮抗了 50%乙醇级分的抗炎作用。
我们确定了康复新液治疗牙周炎的一种有效的抗炎部位（正丁醇提取物的 50%乙醇级分）。该部位抑制了牙周炎样本中 PI3K-AKT-mTOR 信号通路的活性。需要进一步研究以分离和鉴定该部位的特定生物活性化合物。
