Yao Lan, Zhang Wenhua, Wang Xuedong, Guo Lishuang, Liu Wenlu, Li Yueyue, Ma Rui, Hei Yan, Yang Xinji, Zhang Zeyu, Wu Wei
Senior Department of Ophthalmology, 3rd Medical Center of Chinese PLA General Hospital, Beijing 100143, China.
Research Center for Tissue Repair and Regeneration Affiliated to the Medical Innovation Research Division, 4th Medical Center of Chinese PLA General Hospital, Beijing 100048, China.
Bioengineering (Basel). 2024 Sep 14;11(9):922. doi: 10.3390/bioengineering11090922.
Control tissue is essential for ensuring the precision of semiquantitative analysis in back-table fluorescence imaging. However, there remains a lack of agreement on the appropriate selection of control tissues. To evaluate the back-table fluorescence imaging performance of different normal tissues and identify the optimal normal tissue, a cohort of 39 patients with orbital tumors were enrolled in the study. Prior to surgery, these patients received indocyanine green (ICG) and following resection, 43 normal control tissues (34 adipose tissues, 3 skin tissues, 3 periosteal tissues, and 3 muscle tissues) were examined using back-table fluorescence imaging. The skin tissue demonstrated significantly elevated fluorescence intensity in comparison to the diseased tissue, whereas the muscle tissue exhibited a broad range and standard deviation of fluorescence signal intensity. Conversely, the adipose and periosteum displayed weak fluorescence signals with a relatively consistent distribution. Additionally, no significant correlations were found between the signal-to-background ratio (SBR) of adipose tissue and patients' ages, genders, weights, disease duration, tumor origins, dosing of administration of ICG infusion, and the time interval between ICG infusion and surgery. However, a positive correlation was observed between the SBR of adipose tissue and its size, with larger adipose tissues (>1 cm) showing an average SBR 27% higher than smaller adipose tissues (≤1 cm). In conclusion, the findings of this study demonstrated that adipose tissue consistently exhibited homogeneous hypofluorescence during back-table fluorescence imaging, regardless of patient clinical variables or imaging parameters. The size of the adipose tissue was identified as the primary factor influencing its fluorescence imaging characteristics, supporting its utility as an ideal control tissue for back-table fluorescence imaging.
对照组织对于确保手术台上荧光成像半定量分析的准确性至关重要。然而，在对照组织的合适选择上仍存在分歧。为了评估不同正常组织的手术台上荧光成像性能并确定最佳正常组织，本研究纳入了39例眼眶肿瘤患者。手术前，这些患者接受了吲哚菁绿（ICG），切除术后，使用手术台上荧光成像检查了43个正常对照组织（34个脂肪组织、3个皮肤组织、3个骨膜组织和3个肌肉组织）。与病变组织相比，皮肤组织显示出明显升高的荧光强度，而肌肉组织的荧光信号强度范围广且标准差大。相反，脂肪和骨膜显示出较弱的荧光信号，分布相对一致。此外，脂肪组织的信噪比（SBR）与患者的年龄、性别、体重、病程、肿瘤起源、ICG输注给药剂量以及ICG输注与手术之间的时间间隔均未发现显著相关性。然而，观察到脂肪组织的SBR与其大小呈正相关，较大的脂肪组织（>1 cm）的平均SBR比较小的脂肪组织（≤1 cm）高27%。总之，本研究结果表明，在手术台上荧光成像期间，无论患者临床变量或成像参数如何，脂肪组织始终表现出均匀的低荧光。脂肪组织的大小被确定为影响其荧光成像特征的主要因素，支持其作为手术台上荧光成像理想对照组织的实用性。
