Granados-Casas Alan Omar, Fernández-Bravo Ana, Stchigel Alberto Miguel, Cano-Lira José Francisco
Mycology Unit, School of Medicine, Universitat Rovira i Virgili, C/ Sant Llorenç 21, 43201 Reus, Spain.
J Fungi (Basel). 2024 Aug 24;10(9):600. doi: 10.3390/jof10090600.
is a genus within the order (phylum Ascomycota) that includes predominantly saprobic cosmopolitan species. Despite its ability to produce diverse secondary metabolites, no genomic data for spp. are currently available in databases. Therefore, in this study, we obtained, assembled, and annotated the genomic sequence of the ex-type strain of (CBS 219.58). For the genomic sequencing, we employed both the Illumina and PacBio platforms, followed by hybrid assembly using MaSuRCA. Quality assessment of the assembly was performed using QUAST and BUSCO tools. Annotation was conducted using BRAKER2, and functional annotation was completed with InterProScan. The resulting genome was of high quality, with a size of 26.46 Mbp distributed across 38 contigs and a BUSCO completion rate of 95.7%, indicating excellent contiguity and assembly completeness. A total of 8248 protein-encoding genes were predicted, with functional annotations assigned to 73.9% of them. Moreover, 82 genes displayed homology with entries in the Pathogen Host Interactions (PHI) database, while 494 genes exhibited similarity to entries in the Carbohydrate-Active Enzymes (CAZymes) database. Furthermore, 30 biosynthetic gene clusters (BGCs) were identified, suggesting significant potential for the biosynthesis of diverse secondary metabolites. Comparative functional analysis with closely related species unveiled a considerable abundance of domains linked to enzymes involved in keratin degradation, alongside a restricted number of domains associated with enzymes engaged in plant cell wall degradation in all studied species of the . This genome-based elucidation not only enhances our comprehension of the biological characteristics of but also furnishes valuable insights for subsequent investigations concerning species and the order .
是（子囊菌门）目内的一个属，主要包括腐生的世界性物种。尽管它能够产生多种次生代谢产物，但数据库中目前没有 spp. 的基因组数据。因此，在本研究中，我们获得、组装并注释了 （CBS 219.58）模式菌株的基因组序列。对于基因组测序，我们使用了Illumina和PacBio平台，随后使用MaSuRCA进行混合组装。使用QUAST和BUSCO工具对组装进行质量评估。使用BRAKER2进行注释，并使用InterProScan完成功能注释。所得基因组质量很高，大小为26.46 Mbp，分布在38个重叠群中，BUSCO完成率为95.7%，表明具有出色的连续性和组装完整性。总共预测了8248个蛋白质编码基因，其中73.9%被赋予了功能注释。此外，82个基因与病原体宿主相互作用（PHI）数据库中的条目显示出同源性，而494个基因与碳水化合物活性酶（CAZymes）数据库中的条目表现出相似性。此外，还鉴定出30个生物合成基因簇（BGCs），表明其在多种次生代谢产物生物合成方面具有巨大潜力。与密切相关物种的比较功能分析揭示，在所有研究的 物种中，与角蛋白降解相关酶的结构域相当丰富，而与植物细胞壁降解相关酶的结构域数量有限。这种基于基因组的阐释不仅增强了我们对 的生物学特性的理解，也为后续关于 物种和该目的研究提供了有价值的见解。
