Sugarman B J, Aggarwal B B, Hass P E, Figari I S, Palladino M A, Shepard H M
Science. 1985 Nov 22;230(4728):943-5. doi: 10.1126/science.3933111.
Modulation of the growth of human and murine cell lines in vitro by recombinant human tumor necrosis factor-alpha (rTNF-alpha) and recombinant human interferon-gamma (rIFN-gamma) was investigated. rTNF-alpha had cytostatic or cytolytic effects on only some tumor cell lines. When administered together with rIFN-gamma, rTNF-alpha showed enhanced antiproliferative effects on a subset of the cell lines tested. In contrast to its effects on sensitive tumor cells, rTNF-alpha augmented the growth of normal diploid fibroblasts. Variations in the proliferative response induced by rTNF-alpha were apparently not due to differences in either the number of binding sites per cell or their affinity for rTNF-alpha. These observations indicate that the effects of rTNF-alpha on cell growth are not limited to tumor cells, but rather that this protein may have a broad spectrum of activities in vivo.
研究了重组人肿瘤坏死因子-α（rTNF-α）和重组人干扰素-γ（rIFN-γ）对人和鼠细胞系体外生长的调节作用。rTNF-α仅对某些肿瘤细胞系具有细胞抑制或细胞溶解作用。当与rIFN-γ联合使用时，rTNF-α对一部分受试细胞系显示出增强的抗增殖作用。与其对敏感肿瘤细胞的作用相反，rTNF-α促进了正常二倍体成纤维细胞的生长。rTNF-α诱导的增殖反应变化显然不是由于每个细胞的结合位点数量或其对rTNF-α的亲和力存在差异。这些观察结果表明，rTNF-α对细胞生长的作用不限于肿瘤细胞，而是这种蛋白质在体内可能具有广泛的活性。
