Department of Forensic Science, Sam Houston State University, Box 2525, 1003 Bowers Blvd, Huntsville, TX 77341, United States.
Department of Forensic Science, Sam Houston State University, Box 2525, 1003 Bowers Blvd, Huntsville, TX 77341, United States.
J Chromatogr B Analyt Technol Biomed Life Sci. 2024 Oct 15;1247:124303. doi: 10.1016/j.jchromb.2024.124303. Epub 2024 Sep 12.
Novel synthetic opioids are a class of drugs abused for their potent analgesic effect and are responsible for many fatal intoxications, particularly within the United States. A targeted assay was developed and validated using LC-MS/MS, capable of identifying nineteen fentalogs. Solid phase extraction was used to isolate analytes of interest from urine. Limits of detection ranged from 0.05 to 0.1 ng/mL and the limit of quantitation was 0.5 ng/mL. Extraction efficiencies using the optimized procedure were 77-88 % for all targeted species. Bias, precision, matrix effects and interferences were within acceptable thresholds for all analytes. The validated assay was used to identify analytes of interest from thirty-seven individuals that had used fentanyl and related substances. In addition to quantitative analyses, a non-targeted liquid chromatography quadrupole time-of-flight mass spectrometry (LC-Q/TOF-MS) assay was also used to identify additional substances and potential biomarkers. Additional N-oxide and N-dealkylated species were identified using this approach, and the potential for biomarker use is presented, given the stability of some analytes within this class.
新型合成阿片类药物是一类因其强效的镇痛效果而被滥用的药物，它们是许多致命中毒事件的罪魁祸首，尤其是在美国。本研究开发并验证了一种使用 LC-MS/MS 检测的靶向分析方法，该方法能够鉴定 19 种芬太尼类似物。固相萃取用于从尿液中分离感兴趣的分析物。检测限范围为 0.05 至 0.1ng/mL，定量限为 0.5ng/mL。使用优化后的程序，所有目标物质的提取效率均为 77-88%。所有分析物的偏倚、精密度、基质效应和干扰均在可接受范围内。经验证的检测方法用于鉴定 37 名使用过芬太尼和相关物质的个体中的感兴趣分析物。除了定量分析外，还使用非靶向液相色谱-四极杆飞行时间质谱（LC-Q/TOF-MS）检测方法来鉴定其他物质和潜在的生物标志物。通过这种方法还鉴定了额外的 N-氧化物和 N-去烷基化物质，并提出了使用生物标志物的可能性，因为某些分析物在该类别中具有稳定性。
