Neuroprotection Research Laboratories, Departments of Radiology and Neurology, Massachusetts General Hospital and Harvard Medical School, 149 Thirteenth Street, Room 2401, Charlestown, MA, 02129-2000, USA.
Sci Rep. 2024 Sep 27;14(1):22334. doi: 10.1038/s41598-024-72311-4.
The corpus callosum, a major white matter tract in the brain, undergoes age-related functional changes. To extend our investigation of age-related gene expression dynamics in the mouse corpus callosum, we compared RNA-seq data from 2 week-old and 12 week-old wild-type C57BL/6 J mice and identified the differentially expressed genes (e.g., Marcksl1, Chst3, C4b, Neat1, Ndrg1, Emid1, etc.) between these ages. Interestingly, we found that genes highly expressed in myelinating oligodendrocytes were upregulated in 12 week-old mice compared to 2 week-old mice, while genes highly expressed in oligodendrocyte precursor cells (OPCs) and newly formed oligodendrocytes were downregulated. Furthermore, by comparing these genes with the datasets from 20 week-old and 96 week-old mice, we identified novel sets of genes with age-dependent variations in the corpus callosum. These gene expression changes potentially affect key biological pathways and may be closely linked to age-related neurological disorders, including dementia and stroke. Therefore, our results provide an additional dataset to explore age-dependent gene expression dynamics of oligodendrocyte lineage cells in the corpus callosum.
胼胝体是大脑中的主要白质束，它会随着年龄的增长而发生功能变化。为了进一步研究小鼠胼胝体中与年龄相关的基因表达动态变化，我们比较了 2 周龄和 12 周龄野生型 C57BL/6J 小鼠的 RNA-seq 数据，并鉴定了这两个年龄段之间差异表达的基因（例如 Marcksl1、Chst3、C4b、Neat1、Ndrg1、Emid1 等）。有趣的是，我们发现，与 2 周龄相比，在 12 周龄的小鼠中，高度表达于髓鞘形成少突胶质细胞的基因上调，而高度表达于少突胶质前体细胞（OPC）和新形成的少突胶质细胞的基因下调。此外，通过将这些基因与 20 周龄和 96 周龄小鼠的数据集进行比较，我们鉴定了一组在胼胝体中有年龄依赖性变化的新基因。这些基因表达的变化可能会影响关键的生物学途径，并可能与年龄相关的神经退行性疾病（包括痴呆和中风）密切相关。因此，我们的结果提供了一个额外的数据集，用于探索胼胝体中少突胶质细胞谱系细胞的年龄依赖性基因表达动态变化。
