White R L, Spray D C, Campos de Carvalho A C, Wittenberg B A, Bennett M V
Am J Physiol. 1985 Nov;249(5 Pt 1):C447-55. doi: 10.1152/ajpcell.1985.249.5.C447.
Ventricular myocytes were isolated from adult rat hearts using the technique of Wittenberg and Robinson (Cell Tissue Res. 216: 231-251, 1981). These cells exhibited morphology, input resistance, time constant, and excitability expected for cells in intact cardiac tissue. Pairs of these cells were electronically coupled, and junctional conductance was unaffected by transjunctional potential or hyperpolarization of both cells. Brief exposure of cell pairs to medium equilibrated with 100% CO2 or containing 0.1 mM octanol quickly and reversibly decreased junctional conductance. We conclude that gap junctions between pairs of ventricular myocytes possess physiological properties like those of junctions in many other tissues. This preparation will be useful in evaluating drug action on junctional communication in heart.
采用维滕贝格和罗宾逊的技术（《细胞与组织研究》216: 231 - 251, 1981）从成年大鼠心脏中分离出心室肌细胞。这些细胞呈现出完整心脏组织中细胞所预期的形态、输入电阻、时间常数和兴奋性。这些细胞对通过电偶联，且连接电导不受跨连接电位或两个细胞超极化的影响。将细胞对短暂暴露于用100%二氧化碳平衡的培养基或含有0.1 mM辛醇的培养基中，会迅速且可逆地降低连接电导。我们得出结论，成对心室肌细胞之间的间隙连接具有与许多其他组织中的连接相似的生理特性。这种制备方法将有助于评估药物对心脏中连接通讯的作用。
