GENEUINTECH Co., Ltd., Inje University, 197 Injero, Gimhae, Gyeongnam, 50834, Republic of Korea.
Laboratory of Microbiology and Immunology, College of Pharmacy, Inje University, 197 Injero, Gimhae, Gyeongnam, 50834, Republic of Korea.
BMC Biotechnol. 2024 Sep 27;24(1):67. doi: 10.1186/s12896-024-00894-x.
Adenoviruses are commonly utilized as viral vectors for gene therapy, genetic vaccines, and recombinant protein expression. To generate replication-defective adenoviruses, E1-complementing cell lines such as HEK293A are utilized; however, limitations remain. Repeated passage of E1-deleted virus in HEK293A cells increases the occurrence of replication-competent adenoviruses (RCAs). In the present study, we developed a novel cell line originating from human primary cells. L132 cells were transduced two times with E1-encoded retrovirus and three times with E1A-encoded retrovirus. Finally, we selected the most productive L132 cell line for generation of RCA-free adenovirus, GT541. GT541 can serve as an alternative cell line to HEK293A and other adenovirus-producing cells.
腺病毒通常被用作基因治疗、基因疫苗和重组蛋白表达的病毒载体。为了生成复制缺陷型腺病毒，需要使用 E1 互补细胞系，如 HEK293A；然而，仍存在一些限制。在 HEK293A 细胞中反复传代 E1 缺失病毒会增加复制型腺病毒（RCAs）的发生。在本研究中，我们开发了一种源自人原代细胞的新型细胞系。L132 细胞两次转导 E1 编码的逆转录病毒，三次转导 E1A 编码的逆转录病毒。最后，我们选择了最具生产效率的 L132 细胞系来生成无 RCA 的腺病毒 GT541。GT541 可作为 HEK293A 和其他腺病毒生产细胞的替代细胞系。
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