Centro de Investigación en Dinámica Celular, Universidad Autónoma del Estado de Morelos, Cuernavaca, Mexico.
Heinrich Pette Institute, Leibniz Institute for Experimental Virology, Hamburg, Germany.
FEBS Lett. 2019 Dec;593(24):3504-3517. doi: 10.1002/1873-3468.13694. Epub 2019 Dec 8.
The adenovirus E1B 55K (E1B) protein plays major roles in productive adenoviral infection and cellular transformation. Interest in E1B increased because of the potential of adenoviruses as therapeutic vectors, and the E1B gene is commonly deleted from adenovirus vectors for anticancer therapy. E1B activities are spatiotemporally regulated through SUMOylation and phosphorylation, and through interactions with multiple partners that occur presumably at different intracellular sites and times postinfection. E1B is implicated in the formation of viral replication compartments and regulates viral genome replication and transcription, transcriptional repression, degradation of cellular proteins, and several intranuclear steps of viral late mRNA biogenesis. Here, we review advances in our understanding of E1B during productive adenovirus replication and discuss fundamental aspects that remain unresolved.
腺病毒 E1B 55K（E1B）蛋白在有效的腺病毒感染和细胞转化中发挥重要作用。人们对 E1B 的兴趣增加，是因为腺病毒作为治疗性载体的潜力，并且 E1B 基因通常从用于抗癌治疗的腺病毒载体中删除。E1B 的活性通过 SUMO 化和磷酸化以及与多个伙伴的相互作用来进行时空调节，这些相互作用可能发生在感染后不同的细胞内位置和时间。E1B 参与病毒复制区的形成，并调节病毒基因组复制和转录、转录抑制、细胞蛋白降解以及病毒晚期 mRNA 生物发生的几个核内步骤。在这里，我们综述了在有效的腺病毒复制过程中对 E1B 的认识进展，并讨论了一些尚未解决的基本问题。
