LAMP 检测法评估口罩过滤样本，对肺结核检测率高。

Evaluation of testing face-mask filter samples with LAMP shows high rates of detection in pulmonary TB.

机构信息

Respiratory Disease Center, Fukujuji Hospital, Japan Anti-Tuberculosis Association (JATA), Tokyo, Japan;, Department of Basic Mycobacteriosis, Graduate School of Biomedical Science, Nagasaki University, Nagasaki, Japan.

Department of Mycobacterium Reference and Research, Research Institute of Tuberculosis, JATA, Kiyose, Tokyo, Japan.

出版信息

Int J Tuberc Lung Dis. 2024 Oct 1;28(10):476-481. doi: 10.5588/ijtld.24.0190.

DOI:10.5588/ijtld.24.0190

PMID:39334544

Abstract

BACKGROUNDDetection of (MTB) in bioaerosols derived from patients with active pulmonary TB is a potential alternative diagnostic method for patients with presumed TB who cannot expectorate sputum.OBJECTIVETo assess the efficacy of a bioaerosol particle collection method to capture MTB and diagnose TB.METHODSA mask-like filter holder (3D mask) with a water-soluble gelatine filter (GF) and one containing a water-insoluble polypropylene filter (PPF) were prepared. Eligible patients wore the 3D mask with GF or PPF within 3 days of starting anti-TB drugs. The GF and PPF filters were collected after 2 and 8 h. DNA was extracted from the filter samples and tested using loop-mediated isothermal amplification (LAMP).RESULTSFilter samples were collected from 57 and 20 patients with and without active pulmonary TB, respectively. The GF and PPF sensitivity was 76.2% and 83.3%, respectively. The specificity of both methods was 100%. Of the 57 patients diagnosed with non-expectorated sputum samples, including suction phlegm, gastric lavage, and bronchial lavage fluid, 55.6% and 50.0% were positive by GF and PPF, respectively.CONCLUSIONWe present a 3D mask filter sampling method for exhaled bioaerosol particles that can be used in clinical practice to diagnose patients with presumed TB..

摘要

背景从活动性肺结核患者的生物气溶胶中检测分枝杆菌（MTB）是一种潜在的替代诊断方法，适用于那些无法咳出痰液的疑似结核病患者。目的评估一种生物气溶胶颗粒收集方法检测 MTB 和诊断结核病的效果。方法准备了一个带有水溶性明胶滤器（GF）和一个不溶于水的聚丙烯滤器（PPF）的口罩状过滤器支架（3D 口罩）。符合条件的患者在开始抗结核药物治疗后 3 天内佩戴带有 GF 或 PPF 的 3D 口罩。在 2 小时和 8 小时后收集 GF 和 PPF 过滤器。从滤器样本中提取 DNA，并使用环介导等温扩增（LAMP）进行检测。结果分别从活动性肺结核和非活动性肺结核患者中收集了滤器样本。GF 和 PPF 的敏感性分别为 76.2%和 83.3%。两种方法的特异性均为 100%。在诊断为未咳出痰液样本的 57 名患者中，包括抽吸痰液、胃灌洗和支气管灌洗液，GF 和 PPF 的阳性率分别为 55.6%和 50.0%。结论我们提出了一种用于呼出生物气溶胶颗粒的 3D 口罩过滤器采样方法，可在临床实践中用于诊断疑似结核病患者。.