Guangdong Provincial Key Laboratory of Plant Resources Biorefinery, School of Chemical Engineering and Light Industry, Guangdong University of Technology, 100 Waihuan Xi Road, Panyu District, Guangzhou 510006, China.
School of Public Health, Guangzhou Medical University, Guangzhou 511436, China.
Biomacromolecules. 2024 Oct 14;25(10):6624-6634. doi: 10.1021/acs.biomac.4c00775. Epub 2024 Sep 28.
Nitric oxide (NO) has received growing attention as an effective antibacterial agent with broad-spectrum activity and a low risk of resistance. However, it remains challenging to develop effective, controllable, and biocompatible NO-releasing materials. Here, we report a novel NO nanogenerator (AL-BNN6-PEG) self-assembled by lignin, a UV-absorbing and hydrophobic NO donor (,'-di-butyl-,'-dinitroso-1,4-phenylenediamine, BNN6), and PEG-DSPE. It was discovered that upon visible light irradiation (450-460 nm), BNN6 can be decomposed by lignin within micellar nanoparticles via a photoinduced electron transfer mechanism in the aqueous medium. Lignin not only served as a sustainable carrier, enhancing the water dispersity of BNN6, but also acted as a biocompatible photosensitizer, triggering BNN6 decomposition with the concomitant release of NO. As a result, the micellar nanoparticles displayed superior antibacterial effects against Gram-negative and Gram-positive bacteria upon visible light illumination. Moreover, MTT assay revealed the negligible cytotoxic effect of the micellar nanoparticles to the mouse fibroblast cells (L929). This research provides more insight into the BNN6 decomposition mechanism and demonstrates a straightforward, effective, and biocompatible strategy for controlled NO-mediated antibacterial applications.
一氧化氮（NO）作为一种具有广谱活性和低耐药风险的有效抗菌剂，受到了越来越多的关注。然而，开发有效、可控和生物相容的一氧化氮释放材料仍然具有挑战性。在这里，我们报告了一种由木质素、具有紫外吸收和疏水性的一氧化氮供体（1,4-二（正丁基）-1,4-二亚硝基苯二胺，BNN6）和 PEG-DSPE 自组装而成的新型一氧化氮纳米发生器（AL-BNN6-PEG）。研究发现，在可见光照射（450-460nm）下，BNN6 可以通过木质素在胶束纳米颗粒内的光诱导电子转移机制在水介质中分解。木质素不仅作为一种可持续的载体，增强了 BNN6 的水分散性，而且作为一种生物相容的光敏剂，触发 BNN6 分解并伴随一氧化氮的释放。因此，胶束纳米颗粒在可见光照射下对革兰氏阴性菌和革兰氏阳性菌表现出优异的抗菌效果。此外，MTT 试验表明胶束纳米颗粒对小鼠成纤维细胞（L929）的细胞毒性可以忽略不计。这项研究提供了对 BNN6 分解机制的更深入了解，并展示了一种简单、有效和生物相容的策略，用于控制一氧化氮介导的抗菌应用。
