Merino José Joaquín, Parmigiani-Cabaña José María, Parmigiani-Izquierdo José María, Fernández-García Rubén, Cabaña-Muñoz María Eugenia
Facultad de Farmacia, Departamento de Farmacología, Farmacognosia y Botánica, Universidad Complutense de Madrid (UCM), 28040 Madrid, Spain.
Centro de Rehabilitación Oral Multidisciplinaria, 30001 Murcia, Spain.
Antioxidants (Basel). 2024 Aug 29;13(9):1052. doi: 10.3390/antiox13091052.
Psychosocial stress may alter cortisol and/or affect the normal functioning of the immune system. Curcuminoids can promote beneficial effects in neuropsychiatric diseases. We evaluated whether curcumin supplementation for 15 consecutive days (1800 mg/day) would decrease systemic MCP-1, sCD14, and TNF alpha levels in patients with moderate anxiety ( = 81). A total number of 81 subjects were enrolled in this study, divided into the following groups according to their Hamilton scores: a control group including patients without anxiety who were not taking curcumin (Cont, = 22) and an anxiety group including patients with moderate anxiety (Anx, = 22). The curcumin-treated patients experienced moderate anxiety, and they take curcumin for 15 consecutive days (Anx-Cur (after), = 15, 1800 mg/day). An evaluation of 128 patients was conducted, which allowed for their assignment to the study groups according to their scores on Hamilton scale II. The cortisol levels were quantified in salivary samples through ELISA (ng/mL), and malonaldehyde (MDA) levels were measured in plasma via the TBARS assay as an index of lipoperoxidation. Several systemic proinflammatory cytokines (pg/mL: MCP-1, TNF alpha, IL-1 beta) and mediators were quantified through ELISA (pg/mL), including systemic sCD14 levels as a marker of monocyte activation. A two-way bifactorial ANOVA was conducted to evaluate the contributions of the anxiety factor (Anx) and/or curcumin factor (Cur) in all the tested markers, including interactions between both factors. High systemic MCP-1 and elevated sCD14 levels were observed in patients with moderate anxiety, which were reduced with curcumin supplementation. In addition, curcumin prevented cortisol overexpression and decreased MDA levels as an antioxidant response in these patients. Collectively, curcumin presented anti-chemotactic effects by reducing systemic MCP-1 levels in anxiety. Curcumin decreased systemic MCP-1 as well as sCD14 levels in patients with moderate anxiety.
心理社会压力可能会改变皮质醇水平和/或影响免疫系统的正常功能。姜黄素类化合物可对神经精神疾病产生有益影响。我们评估了连续15天补充姜黄素（1800毫克/天）是否会降低中度焦虑患者（n = 81）的全身MCP-1、sCD14和TNF-α水平。本研究共纳入81名受试者，根据他们的汉密尔顿评分分为以下几组：一个对照组，包括未服用姜黄素的无焦虑患者（Cont，n = 22）和一个焦虑组，包括中度焦虑患者（Anx，n = 22）。接受姜黄素治疗的患者患有中度焦虑，他们连续15天服用姜黄素（Anx-Cur（治疗后），n = 15，1800毫克/天）。对128名患者进行了评估，根据他们在汉密尔顿量表II上的得分将他们分配到研究组。通过ELISA（纳克/毫升）对唾液样本中的皮质醇水平进行定量，通过硫代巴比妥酸反应物（TBARS）测定法测量血浆中的丙二醛（MDA）水平作为脂质过氧化指标。通过ELISA（皮克/毫升）对几种全身促炎细胞因子（皮克/毫升：MCP-1、TNF-α、IL-1β）和介质进行定量，包括全身sCD14水平作为单核细胞活化的标志物。进行双向双因素方差分析以评估焦虑因素（Anx）和/或姜黄素因素（Cur）在所有测试标志物中的作用，包括两个因素之间的相互作用。在中度焦虑患者中观察到全身MCP-1水平升高和sCD14水平升高，补充姜黄素后这些水平降低。此外，姜黄素可防止这些患者的皮质醇过度表达，并降低MDA水平作为抗氧化反应。总体而言，姜黄素通过降低焦虑患者的全身MCP-1水平呈现抗趋化作用。姜黄素降低了中度焦虑患者的全身MCP-1以及sCD14水平。
