Tatone Carla, Di Emidio Giovanna, Battaglia Rosalia, Di Pietro Cinzia
Department of Life, Health and Experimental Sciences, University of L'Aquila, 67100 L'Aquila, Italy.
Department of Biomedical and Biotechnological Sciences, Section of Biology and Genetics, University of Catania, 95123 Catania, Italy.
Antioxidants (Basel). 2024 Sep 12;13(9):1101. doi: 10.3390/antiox13091101.
The ovary is a major determinant of female reproductive health. Ovarian functions are mainly related to the primordial follicle pool, which is gradually lost with aging. Ovarian aging and reproductive dysfunctions share oxidative stress as a common underlying mechanism. ROS signaling is essential for normal ovarian processes, yet it can contribute to various ovarian disorders when disrupted. Therefore, balance in the redox system is crucial for proper ovarian functions. In the present study, by focusing on mRNAs and ncRNAs described in the ovary and taking into account only validated ncRNA interactions, we built an ovarian antioxidant ceRNA network, named OvAnOx ceRNA, composed of 5 mRNAs (SOD1, SOD2, CAT, PRDX3, GR), 10 miRNAs and 5 lncRNAs (XIST, FGD5-AS1, MALAT1, NEAT1, SNHG1). Our bioinformatic analysis indicated that the components of OvAnOx ceRNA not only contribute to antioxidant defense but are also involved in other ovarian functions. Indeed, antioxidant enzymes encoded by mRNAs of OvAnOx ceRNA operate within a regulatory network that impacts ovarian reserve, follicular dynamics, and oocyte maturation in normal and pathological conditions. The OvAnOx ceRNA network represents a promising tool to unravel the complex dialog between redox potential and ovarian signaling pathways involved in reproductive health, aging, and diseases.
卵巢是女性生殖健康的主要决定因素。卵巢功能主要与原始卵泡池有关，而原始卵泡池会随着年龄增长逐渐减少。卵巢衰老和生殖功能障碍都有氧化应激这一共同的潜在机制。ROS信号传导对正常卵巢过程至关重要，但当它被破坏时会导致各种卵巢疾病。因此，氧化还原系统的平衡对于卵巢正常功能至关重要。在本研究中，通过关注卵巢中描述的mRNA和ncRNA，并仅考虑经过验证的ncRNA相互作用，我们构建了一个卵巢抗氧化ceRNA网络，名为OvAnOx ceRNA，它由5种mRNA（SOD1、SOD2、CAT、PRDX3、GR）、10种miRNA和5种lncRNA（XIST、FGD5-AS1、MALAT1、NEAT1、SNHG1）组成。我们的生物信息学分析表明，OvAnOx ceRNA的组成成分不仅有助于抗氧化防御，还参与其他卵巢功能。事实上，OvAnOx ceRNA的mRNA编码的抗氧化酶在一个调节网络中发挥作用，该网络在正常和病理条件下影响卵巢储备、卵泡动态和卵母细胞成熟。OvAnOx ceRNA网络是一个很有前途的工具，可用于揭示氧化还原电位与参与生殖健康、衰老和疾病的卵巢信号通路之间的复杂对话。
