Khalil Atif Ali Khan, Woo Min-Seok, Kang Dong-Min, Ahn Mi-Jeong, Kim Jeong-Ah, Yang Heejung, Kim Jung-Hwan
Department of Pharmacology, Institute of Health Sciences, School of Medicine, Gyeongsang National University, Jinju 52727, Republic of Korea.
Research Institute of Pharmaceutical Sciences, College of Pharmacy, Gyeongsang National University, Jinju 52828, Republic of Korea.
Antioxidants (Basel). 2024 Sep 16;13(9):1119. doi: 10.3390/antiox13091119.
L., a member of the Anacardiaceae family, is widely cultivated across the globe. The leaves of are renowned for their medicinal properties, attributed to the abundance of bioactive compounds. This study investigated the effects of mango leaf extract on oxidative stress in HeLa cells. Notably, the n-hexane fraction (MLHx) significantly enhanced antioxidant response element (ARE)-luciferase activity at a concentration of 100 µg/mL, surpassing other fractions. MLHx also promoted the expression of HO-1 mRNA by increasing nuclear NRF2 levels. The molecular mechanism of MLHx involves increased phosphorylation of ERK1/2 and stabilization of NRF2. Bioactivity-guided isolation resulted in the identification of six oxylipins: 13(R)-hydroxy-octadeca-(9Z,11E,15Z)-trienoic acid (), 9(R)-hydroxy-octadeca-(10E,12Z,15Z)-trienoic acid (), 13(R)-hydroxy-(9Z,11E)-octadecadienoic acid (), 9(R)-hydroxy-(10E,12Z)-octadecadienoic acid (), 9-oxo-(10E,12E)-octadecadienoic acid (), and 9-oxo-(10E,12Z)-octadecadienoic acid (). These structures were elucidated using comprehensive spectroscopic techniques, including MS and 1H NMR. Additionally, compounds (9-oxo-(10E,12Z,15Z)-octadecatrienoic acid) and (13-oxo-(9E,11E)-octadecadienoic acid) were characterized by LC-MS/MS mass fragmentation. This study reports the isolation of compounds 1-6 from for the first time. When tested for their effect on NRF2 activity in HeLa cells, compounds , , and showed strong stimulation of ARE-luciferase activity in a dose-dependent manner.
芒果属于漆树科，在全球广泛种植。芒果叶因其富含生物活性化合物而具有药用特性而闻名。本研究调查了芒果叶提取物对HeLa细胞氧化应激的影响。值得注意的是，正己烷馏分（MLHx）在浓度为100μg/mL时显著增强了抗氧化反应元件（ARE）-荧光素酶活性，超过了其他馏分。MLHx还通过增加核NRF2水平促进了HO-1 mRNA的表达。MLHx的分子机制涉及ERK1/2磷酸化增加和NRF2的稳定。生物活性导向分离鉴定出六种氧化脂质：13（R）-羟基-十八碳-（9Z，11E，15Z）-三烯酸、9（R）-羟基-十八碳-（10E，12Z，15Z）-三烯酸、13（R）-羟基-（9Z，11E）-十八碳二烯酸、9（R）-羟基-（10E，12Z）-十八碳二烯酸、9-氧代-（10E，12E）-十八碳二烯酸和9-氧代-（10E，12Z）-十八碳二烯酸。这些结构通过包括MS和1H NMR在内的综合光谱技术得以阐明。此外，化合物（9-氧代-（10E，12Z，15Z）-十八碳三烯酸）和（13-氧代-（9E，11E）-十八碳二烯酸）通过LC-MS/MS质谱裂解进行了表征。本研究首次报道了从芒果中分离出化合物1-6。当测试它们对HeLa细胞中NRF2活性的影响时，化合物、和以剂量依赖方式强烈刺激了ARE-荧光素酶活性。
