Center for Nanomedicine and Theranostics, Department of Health Technology, Technical University of Denmark, Nils Koppels Allé, Building 423, 2800 Kongens Lyngby, Denmark.
Biomolecules. 2024 Aug 23;14(9):1046. doi: 10.3390/biom14091046.
The growing demand for effective alternatives to red blood cells (RBCs) has spurred significant research into hemoglobin (Hb)-based oxygen carriers (HBOCs). Accurate characterization of HBOCs-including Hb content, encapsulation efficiency, and yield-is crucial for ensuring effective oxygen delivery, economic viability, and the prevention of adverse effects caused by free Hb. However, the choice of quantification methods for HBOCs is often driven more by tradition than by a thorough assessment of available options. This study meticulously compares various UV-vis spectroscopy-based methods for Hb quantification, focusing on their efficacy in measuring Hb extracted from bovine RBCs across different concentration levels. The findings identify the sodium lauryl sulfate Hb method as the preferred choice due to its specificity, ease of use, cost-effectiveness, and safety, particularly when compared to cyanmethemoglobin-based methods. Additionally, the study discusses the suitability of these methods for HBOC characterization, emphasizing the importance of considering carrier components and potential interferences by analyzing the absorbance spectrum before selecting a method. Overall, this study provides valuable insights into the selection of accurate and reliable Hb quantification methods, which are essential for rigorous HBOC characterization and advancements in medical research.
对红细胞（RBC）有效替代品的需求不断增长，这促使人们对基于血红蛋白（Hb）的氧载体（HBOCs）进行了大量研究。准确表征 HBOCs，包括 Hb 含量、包封效率和得率，对于确保有效供氧、经济可行性以及预防游离 Hb 引起的不良反应至关重要。然而，HBOCs 的定量方法的选择往往更多地受到传统的驱动，而不是对可用选项进行彻底评估。本研究细致比较了基于紫外-可见分光光度法的各种 Hb 定量方法，重点关注它们在测量不同浓度水平下从牛 RBC 中提取的 Hb 的效果。研究结果表明，由于其特异性、易用性、成本效益和安全性，特别是与氰化高铁血红蛋白法相比，月桂基硫酸钠 Hb 法是首选方法。此外，该研究还讨论了这些方法在 HBOC 表征中的适用性，强调在选择方法之前，通过分析吸收光谱来考虑载体成分和潜在干扰的重要性。总的来说，本研究为选择准确可靠的 Hb 定量方法提供了有价值的见解，这对于严格的 HBOC 表征和医学研究的进展至关重要。
