a School of Environmental and Chemical Engineering , Tianjin Polytechnic University , Tianjin , P. R. China.
b State Key Laboratory of Separation Membranes and Membrane Processes , Tianjin Polytechnic University , Tianjin , P. R. China.
Artif Cells Nanomed Biotechnol. 2018;46(sup3):S1076-S1082. doi: 10.1080/21691401.2018.1529677. Epub 2018 Nov 19.
Red blood cells (RBCs) possess intact cyto-architectures while haemoglobin (Hb) is a cell-free, homogeneous solution. Both RBCs and Hb are generalized oxygen carriers. In this paper, kinetic studies on oxygen-releasing of high concentration of Hb and RBCs under various conditions were carried out regarding Hb and RBCs as fluids. Among them, Hb under specific conditions was seen as the simplest Hb-based oxygen carrier (HBOC), Also, factors affecting the oxygen releasing of Hb and RBCs, including osmotic pressure, viscosity and allosteric agent, have been well studied. Analysis of the results from the measurement above showed that kinetics of oxygen releasing of either pure Hb or the simplest HBOCs was obviously different from that of RBCs. The oxygen-releasing time of Hb was shorter and the oxygen-releasing rates of Hb were quicker than those of RBCs under various conditions. Therefore, as fluids, only by changing the milieus it exists in, Hb could not achieve the expected oxygen-releasing effect on the microcirculation so well as RBCs do in the same system, irrespective of the interaction between the fluids and blood vessels. Furthermore, kinetic properties of HBOCs must be considered and matched with those of RBCs in the study of HBOCs.
红细胞 (RBC) 具有完整的细胞结构，而血红蛋白 (Hb) 是一种无细胞的均相溶液。RBC 和 Hb 都是通用的氧载体。在本文中，将 Hb 和 RBC 视为流体，对高浓度 Hb 和 RBC 在各种条件下的释氧动力学进行了研究。其中，在特定条件下的 Hb 被视为最简单的基于 Hb 的氧载体 (HBOC)。此外，还研究了影响 Hb 和 RBC 释氧的因素，包括渗透压、粘度和变构剂。对上述测量结果的分析表明，无论是纯 Hb 还是最简单的 HBOCs 的释氧动力学明显不同于 RBCs。在各种条件下，Hb 的释氧时间更短，释氧速率也比 RBCs 更快。因此，作为流体，Hb 无法像 RBCs 那样在相同的系统中通过改变其所处的环境来实现对微循环的预期释氧效果，而不管流体与血管之间的相互作用如何。此外，在 HBOCs 的研究中，必须考虑 HBOCs 的动力学特性，并使其与 RBCs 的动力学特性相匹配。
