Guidez Amandine, Tirera Sourakhata, Talaga Stanislas, Lacour Guillaume, Carinci Romuald, Darcissac Edith, Donato Damien, Gaborit Pascal, Clervil Emmanuelle, Epelboin Yanouk, de Thoisy Benoit, Dusfour Isabelle, Duchemin Jean-Bernard, Lavergne Anne
Unité d'Entomologie Médicale, Institut Pasteur de la Guyane, 97354 Cayenne, France.
Laboratoire des Interactions Virus-Hôtes, Institut Pasteur de la Guyane, 97354 Cayenne, France.
Insects. 2024 Sep 19;15(9):718. doi: 10.3390/insects15090718.
Pathogens transmitted by mosquitoes (Diptera, Culicidae) in sylvatic or urban cycles involve wild or domestic animals and humans, driven by various mosquito species with distinct host preferences. Understanding mosquito-host associations is crucial for ecological insights and pathogen surveillance. In this study, we analyzed mosquito blood meals from coastal French Guiana by amplifying and sequencing host DNA from blood-fed females. Using the 12S ribosomal RNA gene and Sanger sequencing, we identified blood meals from 26 mosquito species across six genera, with 59% belonging to the genus. Nanopore sequencing of selected samples showed 12 mosquito species with one to three mixed blood-meal sources. Mammals were the primary hosts (88%), followed by birds (7%), squamates (3%), and amphibians (2%), indicating a strong preference for mammalian hosts. A total of 46 vertebrate host species were identified, demonstrating high host diversity. This research provides insights into mosquito host usage and highlights the complexities of monitoring arboviruses of public health concern.
在野生或城市环境中，由蚊子（双翅目，蚊科）传播的病原体涉及野生动物、家畜和人类，传播媒介是具有不同宿主偏好的各种蚊子。了解蚊子与宿主的关联对于生态学研究和病原体监测至关重要。在本研究中，我们通过对吸食血液的雌蚊体内的宿主DNA进行扩增和测序，分析了法属圭亚那沿海地区蚊子的血餐。利用12S核糖体RNA基因和桑格测序法，我们鉴定出了六个属的26种蚊子的血餐，其中59%属于该属。对选定样本的纳米孔测序显示，有12种蚊子的血餐来源有一到三种混合情况。哺乳动物是主要宿主（88%），其次是鸟类（7%）、有鳞目动物（3%）和两栖动物（2%），表明蚊子对哺乳动物宿主有强烈偏好。总共鉴定出46种脊椎动物宿主物种，显示出高度的宿主多样性。这项研究为了解蚊子的宿主利用情况提供了见解，并突出了监测公共卫生相关虫媒病毒的复杂性。
