Yamada Hanano, Hood-Nowotny Rebecca, Resch Christian, Bouyer Jeremy, Gruber Roman, Oliva Clelia F
Insect Pest Control Laboratory, Joint FAO/IAEA Centre of Nuclear Techniques in Food and Agriculture, International Atomic Energy Agency, Vienna International Centre, P.O. Box 100, 1400 Vienna, Austria.
Department of Forest and Soil Sciences, Institute of Soil Research, University of Natural Resources and Life Sciences Vienna, Konrad-Lorenz-Strasse 24, 3430 Tulln, Austria.
Insects. 2024 Sep 20;15(9):721. doi: 10.3390/insects15090721.
The key to success in the application of the sterile insect technique (SIT) relies on the ability of released, sterile males to outcompete their fertile wild male counterparts to mate with wild females. However, many insect species exhibit multiple-mating behavior, which can be a way for females to select paternity for their progeny. This study aims to recognize the consequences of potential double-matings during an SIT program and to detect any evidence of sperm selection favoring sperm from fertile mates. This report provides a descriptive analysis of the storage and use of sperm by female Stable isotopes were used to mark the sperm of fertile and sterile males. Mated females were allowed to oviposit before dissecting the spermathecae to link the presence of each type of sperm to the sterility of the eggs laid. It was found that sperm in females inseminated by both males was distributed in the three spermathecae with no obvious pattern, mostly mixed but also separately, and no evidence of any mechanism for sperm selection, sperm precedence, or sperm competition in females could be found. The fact that only a few double-mated females were double-inseminated and could also produce semi-sterile eggs, together with the finding that the sperm of sterile males appeared to be no less viable than that of fertile males, is an encouraging outcome for SIT approaches.
应用昆虫不育技术（SIT）取得成功的关键在于，释放的不育雄虫能够比可育的野生雄虫更具竞争力，从而与野生雌虫交配。然而，许多昆虫物种表现出多次交配行为，这可能是雌虫为其后代选择父本的一种方式。本研究旨在认识昆虫不育技术项目中潜在的双重交配的后果，并检测是否有任何证据表明雌虫会选择可育配偶的精子。本报告对雌虫精子的储存和使用进行了描述性分析。使用稳定同位素标记可育和不育雄虫的精子。让已交配的雌虫产卵，然后解剖受精囊，以将每种类型精子的存在与所产卵的不育情况联系起来。研究发现，由两种雄虫授精的雌虫体内的精子在三个受精囊中分布没有明显规律，大多是混合的，但也有分开的情况，并且未发现雌虫存在任何精子选择、精子优先或精子竞争机制的证据。只有少数双重交配的雌虫是双重授精的，并且还能产出半不育卵，再加上不育雄虫的精子似乎与可育雄虫的精子活力相当这一发现，对于昆虫不育技术方法来说是一个令人鼓舞的结果。
