游离细胞 DNA 羟甲基化在癌症中的应用：当前和新兴的检测方法及临床应用。
Cell-Free DNA Hydroxymethylation in Cancer: Current and Emerging Detection Methods and Clinical Applications.
机构信息
Department of Medical Biophysics, Temerty Faculty of Medicine, University of Toronto, Princess Margaret Cancer Research Tower, 101 College Street, Room 9-309, Toronto, ON M5G 1L7, Canada.
Institute of Medical Science, Temerty Faculty of Medicine, University of Toronto, 1 King's College Circle, Medical Sciences Building, Room 2374, Toronto, ON M5S 1A8, Canada.
出版信息
Genes (Basel). 2024 Sep 3;15(9):1160. doi: 10.3390/genes15091160.
In the era of precision oncology, identifying abnormal genetic and epigenetic alterations has transformed the way cancer is diagnosed, managed, and treated. 5-hydroxymethylcytosine (5hmC) is an emerging epigenetic modification formed through the oxidation of 5-methylcytosine (5mC) by ten-eleven translocase (TET) enzymes. DNA hydroxymethylation exhibits tissue- and cancer-specific patterns and is essential in DNA demethylation and gene regulation. Recent advancements in 5hmC detection methods and the discovery of 5hmC in cell-free DNA (cfDNA) have highlighted the potential for cell-free 5hmC as a cancer biomarker. This review explores the current and emerging techniques and applications of DNA hydroxymethylation in cancer, particularly in the context of cfDNA.
在精准肿瘤学时代，识别异常的遗传和表观遗传改变已经改变了癌症的诊断、管理和治疗方式。5-羟甲基胞嘧啶（5hmC）是一种新兴的表观遗传修饰，通过 TET 酶将 5-甲基胞嘧啶（5mC）氧化形成。DNA 羟甲基化表现出组织和癌症特异性模式，对于 DNA 去甲基化和基因调控至关重要。最近在 5hmC 检测方法上的进展以及在游离细胞 DNA（cfDNA）中发现 5hmC，突显了游离细胞 5hmC 作为癌症生物标志物的潜力。本综述探讨了 DNA 羟甲基化在癌症中的当前和新兴技术及应用，特别是在 cfDNA 方面。
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